LOCUS       RR215040                7683 bp    DNA     circular  SYN 
DEFINITION  Pign (Myc-DDK-tagged ORF) - Rat phosphatidylinositol glycan, class N (Pign), (10 ug)
ACCESSION   NM_001100584
VERSION     NM_001100584.1
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    3838..3929 
                     /label="Myc-DDK"  
     misc_feature    3959..4544 
                     /label="PolyA signal"  
     misc_feature    4016..4035 
                     /label="Restriction Enzyme"  
     misc_feature    4820..5492 
                     /label="ColE1"  
     misc_feature    5922..6716 
                     /label="Kan/Neo"  
     misc_feature    6751..7053 
                     /label="SV40 ori"  
     misc_feature    7139..7616 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    3813..3820 
                     /label="Restriction Enzyme"  
     misc_feature    1020..3813 
                     /label="Pign"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GCTGCTGTTT TTTGCTTTGG GATTGCTTGT GCATTTTGTG TTCTTCGCCT 
     1081 CCATCTTTGA CATTTATTTT ACCTCTCCTT TGGTTCATGG AATGACTCCT CAGGTTACAC 
     1141 CATTGCCTCC TCCAGCAAAA AGATTAGTGT TATTTGTTGC TGACGGCCTG AGAGCAGATG 
     1201 CTCTTTATGA GTTAGATGAA GATGGAAACT CCAGAGCACC TTTTATTAGG AATGTTATCA 
     1261 TGCATGAAGG CAGCTGGGGA GTCTCTCACA CGCGTGTGCC AACAGAGTCT CGGCCTGGCC 
     1321 ACGTAGCCTT GATAGCTGGG TTTTATGAGG ATGTCAGTGC AGTCGCCAAA GGGTGGAAAG 
     1381 AAAATCCTGT AGAATTTGAT TCTCTTTTTA ATGAAAGCAA GTACACATGG AGCTGGGGGA 
     1441 GTCCAGATAT CTTGCCTATG TTTGCCAAAG GTGCTAGTGG TGACCATGTT TATACTTACA 
     1501 GTTACGATGC TCAAAGAGAA GATTTTGGTG CTCACGATGC CACAAAGCTG GATACCTGGG 
     1561 TTTTTGATAA AGTTAAGGAC TTCTTTGATG CTGCAAGAAA CAACCAATCT TTGTTCTCAA 
     1621 TAGTAAATGA AGAAAAAGTC GTTTTTTTCT TACATTTATT AGGAATAGAT ACAAATGGAC 
     1681 ATGCTCATCG ACCATCATCA AGGGAATATA AGGACAATAT TAAAAAAGTT GATGATGGAG 
     1741 TGAAGGAAAT TGTATCAATA TTCAAGCATT TTTATGAAGA TGATGGGAAA ACAGCATTTA 
     1801 TTTTTACCTC TGACCATGGA ATGACAGACT GGGGTTCCCA TGGGGCTGGT CATCCTTCAG 
     1861 AGACTTTAAC TCCTTTCGTT ACTTGGGGAG CTGGAATCAA ATTTCCTCAA AATGTATCAG 
     1921 CTCAGCAATA TGAGGATGAG TTTTTAAAAG AATGGAGATT GGAAAATTGG AAGAGGCAAG 
     1981 ATATCAATCA GGCTGATATT GCACCACTGA TGGCATCCCT TATTGGAGTC CCCTTTCCTC 
     2041 TTAATTCGGT GGGAATACTT CCTGTGGGTT ATCTTAACAA CACTGGTCTC TTCAAAGCAG 
     2101 AGAGCATGTT TACCAATGCA GTGCAGATTC TTGAACAGTT CAAGGTGAAA ATGACTCAGA 
     2161 AAAAAGAAGC TACTTTACCA TTTTTGTTTA CACCATTTAA GTTGCTCTCT GATTCTCAAC 
     2221 AGCTGGACAT TTTAAGAAAA GCAAGATCTT ACATAAAACA AGAAAAATTT GATGACGTAG 
     2281 TGTCCCTCTG TGAGGAGCTG ATTGATCTGG CCCTGAGAGG ATTGTCCTAT TATCACACTT 
     2341 ACGACAGACT GTTTCTGGGT ATCAGTGTTG CAGTTGGGTT TGTGGGATGG ACATCGTATG 
     2401 CTTCCTTGTT AATCATCAAG TCTCATTCTG ATATCCCACG AGGCACTAGG AGGGAAGGGA 
     2461 AGAAGCCACA CCATCTTCTG CCCTGTAGCT TCATAGCTGC TGGTGTCTTA GTAGCATGCT 
     2521 TCCTGATGAT CCAAGCCTGT CCCTGGACTT ACTATGTGTA TTGCTTGCTG CCGGTGCCAA 
     2581 TATGGTATGC AGTTCTAAAA GAACGTGAAG TTATTCAAGC CCTGGTTGAG TCACTATTGG 
     2641 CTTTTCCTCT GAGCCGTTTT GTCGCCTACT TGCTTGTCTT TACCCTGGGC ATTGAAGTAC 
     2701 TGGTGCTCAG TTTTTTCTAC CGCTACATGT TGACAGCTGG ACTGATTGTG TTTGCAGGTT 
     2761 GGCCATTTCT CACTCAGCTG TGGACCCGAG CAAAGATCAC CTGCCTGAGT TGGGCTTTCT 
     2821 TCTCTTTGCT TCTGGCAGTG TTCCCACTGA TGCCTGTTGT GGGTAGAAAG CCAGACCTTT 
     2881 CTCTAGTAAT GGGTGCCGGA TTGCTTGTTC TTCTGCTGTC GCTGGCTGTT CTAGCAACCC 
     2941 TCGTAAAAAG AAAACTTAAT TTGGTAAAGG ACGAGCTGTT GGTACTTCTG TTACAGGTGC 
     3001 TGAGTACAGT GCTCTCTATG TACGTTGTGT ACAGTACCCA CCACAGTCTG TTAAAGAAAG 
     3061 AAGGCCTGCC TCTTCTGAAT CAGATTGTGA GCTGGGCAAC GCTAGCCTCT TCTCTGGTGG 
     3121 TGCCTCTCCT GAGCCCCACA GCCCTCTGGC AGCGGCTGAC CAGCATACTT CTTTCCTTGA 
     3181 TGTCGACCTA CTTGCTGCTA AGCACGGGGT ATGAAGCTCT CTTTCCGTTA GTGTTGTCCT 
     3241 GTTTGATGTT TGTCTGGATA CACGTGGAAC AAGAGACCCT TCAACAGTCT GGTGTTTCCT 
     3301 GTAAACAAAA GCTTGCCAGT ATCCAGTTCA CCTACAATAC TGATATAACT CAGTTCCGAC 
     3361 AGCTCTGTTT GGATGACATC CGTAGGGCCT TTTTCCTTGT TTTCTTTTTA TTAACTGCAT 
     3421 TTTTTGGAAC TGGAAATATA GCTTCTATTA ACAGCTTTGA CCTTGCCTCT GTGTATTGCT 
     3481 TTCTGACTGT GTTTAGTCCT TTCATGATGG GAGCGCTCAT GATGTGGAAG ATTTTAATTC 
     3541 CTTTTGTTCT TGTGATGTGT GCTTTTGAAG CAGTTCAGAT AACTACACAG CTGTCATCAA 
     3601 AAGGCCTTTT TCTTGTCGTT CTTGTCATAT CAGACATCAT GGCTTTGCAT TTTTTCTTCT 
     3661 TGGTCAAGGA TTCTGGCAGC TGGCTTGATA TTGGAACAAG CATCAGCCAC TATGTGATTG 
     3721 TCATGTCCAT GACCATCTTT CTGGTGTTTC TTAATGGCCT GGCACAATTG CTCACAACAA 
     3781 AGAAACTCCA ACTGTGTGGC AAGCCCAAAA GCCATCTCAT GAGCGGACCG ACGCGTACGC 
     3841 GGCCGCTCGA GCAGAAACTC ATCTCAGAAG AGGATCTGGC AGCAAATGAT ATCCTGGATT 
     3901 ACAAGGATGA CGACGATAAG GTTTAAACGG CCGGCCGCGG TCATAGCTGT TTCCTGAACA 
     3961 GATCCCGGGT GGCATCCCTG TGACCCCTCC CCAGTGCCTC TCCTGGCCCT GGAAGTTGCC 
     4021 ACTCCAGTGC CCACCAGCCT TGTCCTAATA AAATTAAGTT GCATCATTTT GTCTGACTAG 
     4081 GTGTCCTTCT ATAATATTAT GGGGTGGAGG GGGGTGGTAT GGAGCAAGGG GCAAGTTGGG 
     4141 AAGACAACCT GTAGGGCCTG CGGGGTCTAT TGGGAACCAA GCTGGAGTGC AGTGGCACAA 
     4201 TCTTGGCTCA CTGCAATCTC CGCCTCCTGG GTTCAAGCGA TTCTCCTGCC TCAGCCTCCC 
     4261 GAGTTGTTGG GATTCCAGGC ATGCATGACC AGGCTCAGCT AATTTTTGTT TTTTTGGTAG 
     4321 AGACGGGGTT TCACCATATT GGCCAGGCTG GTCTCCAACT CCTAATCTCA GGTGATCTAC 
     4381 CCACCTTGGC CTCCCAAATT GCTGGGATTA CAGGCGTGAA CCACTGCTCC CTTCCCTGTC 
     4441 CTTCTGATTT TAAAATAACT ATACCAGCAG GAGGACGTCC AGACACAGCA TAGGCTACCT 
     4501 GGCCATGCCC AACCGGTGGG ACATTTGAGT TGCTTGCTTG GCACTGTCCT CTCATGCGTT 
     4561 GGGTCCACTC AGTAGATGCC TGTTGAATTG GGTACGCGGC CAGCGGCGAG CGGTATCAGC 
     4621 TCACTCAAAG GCGGTAATAC GGTTATCCAC AGAATCAGGG GATAACGCAG GAAAGAACAT 
     4681 GTGAGCAAAA GGCCAGCAAA AGGCCAGGAA CCGTAAAAAG GCCGCGTTGC TGGCGTTTTT 
     4741 CCATAGGCTC CGCCCCCCTG ACGAGCATCA CAAAAATCGA CGCTCAAGTC AGAGGTGGCG 
     4801 AAACCCGACA GGACTATAAA GATACCAGGC GTTTCCCCCT GGAAGCTCCC TCGTGCGCTC 
     4861 TCCTGTTCCG ACCCTGCCGC TTACCGGATA CCTGTCCGCC TTTCTCCCTT CGGGAAGCGT 
     4921 GGCGCTTTCT CATAGCTCAC GCTGTAGGTA TCTCAGTTCG GTGTAGGTCG TTCGCTCCAA 
     4981 GCTGGGCTGT GTGCACGAAC CCCCCGTTCA GCCCGACCGC TGCGCCTTAT CCGGTAACTA 
     5041 TCGTCTTGAG TCCAACCCGG TAAGACACGA CTTATCGCCA CTGGCAGCAG CCACTGGTAA 
     5101 CAGGATTAGC AGAGCGAGGT ATGTAGGCGG TGCTACAGAG TTCTTGAAGT GGTGGCCTAA 
     5161 CTACGGCTAC ACTAGAAGAA CAGTATTTGG TATCTGCGCT CTGCTGAAGC CAGTTACCTT 
     5221 CGGAAAAAGA GTTGGTAGCT CTTGATCCGG CAAACAAACC ACCGCTGGTA GCGGTGGTTT 
     5281 TTTTGTTTGC AAGCAGCAGA TTACGCGCAG AAAAAAAGGA TCTCAAGAAG ATCCTTTGAT 
     5341 CTTTTCTACG GGGTCTGACG CTCAGTGGAA CGAAAACTCA CGTTAAGGGA TTTTGGTCAT 
     5401 GAGATTATCA AAAAGGATCT TCACCTAGAT CCTTTTAAAT TAAAAATGAA GTTTTAAATC 
     5461 AATCTAAAGT ATATATGAGT AACCTGAGGC TATGGCAGGG CCTGCCGCCC CGACGTTGGC 
     5521 TGCGAGCCCT GGGCCTTCAC CCGAACTTGG GGGGTGGGGT GGGGAAAAGG AAGAAACGCG 
     5581 GGCGTATTGG CCCCAATGGG GTCTCGGTGG GGTATCGACA GAGTGCCAGC CCTGGGACCG 
     5641 AACCCCGCGT TTATGAACAA ACGACCCAAC ACCGTGCGTT TTATTCTGTC TTTTTATTGC 
     5701 CGTCATAGCG CGGGTTCCTT CCGGTATTGT CTCCTTCCGT GTTTCAGTTA GCCTCCCCCT 
     5761 AGGGTGGGCG AAGAACTCCA GCATGAGATC CCCGCGCTGG AGGATCATCC AGCCGGCGTC 
     5821 CCGGAAAACG ATTCCGAAGC CCAACCTTTC ATAGAAGGCG GCGGTGGAAT CGAAATCTCG 
     5881 TGATGGCAGG TTGGGCGTCG CTTGGTCGGT CATTTCGAAC CCCAGAGTCC CGCTCAGAAG 
     5941 AACTCGTCAA GAAGGCGATA GAAGGCGATG CGCTGCGAAT CGGGAGCGGC GATACCGTAA 
     6001 AGCACGAGGA AGCGGTCAGC CCATTCGCCG CCAAGCTCTT CAGCAATATC ACGGGTAGCC 
     6061 AACGCTATGT CCTGATAGCG ATCCGCCACA CCCAGCCGGC CACAGTCGAT GAATCCAGAA 
     6121 AAGCGGCCAT TTTCCACCAT GATATTCGGC AAGCAGGCAT CGCCATGGGT CACGACGAGA 
     6181 TCCTCGCCGT CGGGCATGCT CGCCTTGAGC CTGGCGAACA GTTCGGCTGG CGCGAGCCCC 
     6241 TGATGCTCTT CGTCCAGATC ATCCTGATCG ACAAGACCGG CTTCCATCCG AGTACGTGCT 
     6301 CGCTCGATGC GATGTTTCGC TTGGTGGTCG AATGGGCAGG TAGCCGGATC AAGCGTATGC 
     6361 AGCCGCCGCA TTGCATCAGC CATGATGGAT ACTTTCTCGG CAGGAGCAAG GTGAGATGAC 
     6421 AGGAGATCCT GCCCCGGCAC TTCGCCCAAT AGCAGCCAGT CCCTTCCCGC TTCAGTGACA 
     6481 ACGTCGAGCA CAGCTGCGCA AGGAACGCCC GTCGTGGCCA GCCACGATAG CCGCGCTGCC 
     6541 TCGTCTTGCA GTTCATTCAG GGCACCGGAC AGGTCGGTCT TGACAAAAAG AACCGGGCGC 
     6601 CCCTGCGCTG ACAGCCGGAA CACGGCGGCA TCAGAGCAGC CGATTGTCTG TTGTGCCCAG 
     6661 TCATAGCCGA ATAGCCTCTC CACCCAAGCG GCCGGAGAAC CTGCGTGCAA TCCATCTTGT 
     6721 TCAATCATGC GAAACGATCC TCATCCTGTC TCTTGATCGA TCTTTGCAAA AGCCTAGGCC 
     6781 TCCAAAAAAG CCTCCTCACT ACTTCTGGAA TAGCTCAGAG GCCGAGGCGG CCTCGGCCTC 
     6841 TGCATAAATA AAAAAAATTA GTCAGCCATG GGGCGGAGAA TGGGCGGAAC TGGGCGGAGT 
     6901 TAGGGGCGGG ATGGGCGGAG TTAGGGGCGG GACTATGGTT GCTGACTAAT TGAGATGCAT 
     6961 GCTTTGCATA CTTCTGCCTG CTGGGGAGCC TGGGGACTTT CCACACCTGG TTGCTGACTA 
     7021 ATTGAGATGC ATGCTTTGCA TACTTCTGCC TGCTGGGGAG CCTGGGGACT TTCCACACCC 
     7081 TAACTGACAC ACATTCCACA GCTGGTTCTT TCCGCCTCAG GACTCTTCCT TTTTCAATAT 
     7141 TATTGAAGCA TTTATCAGGG TTATTGTCTC ATGAGCGGAT ACATATTTGA ATGTATTTAG 
     7201 AAAAATAAAC AAATAGGGGT TCCGCGCACA TTTCCCCGAA AAGTGCCACC TGACGCGCCC 
     7261 TGTAGCGGCG CATTAAGCGC GGCGGGTGTG GTGGTTACGC GCAGCGTGAC CGCTACACTT 
     7321 GCCAGCGCCC TAGCGCCCGC TCCTTTCGCT TTCTTCCCTT CCTTTCTCGC CACGTTCGCC 
     7381 GGCTTTCCCC GTCAAGCTCT AAATCGGGGG CTCCCTTTAG GGTTCCGATT TAGTGCTTTA 
     7441 CGGCACCTCG ACCCCAAAAA ACTTGATTAG GGTGATGGTT CACGTAGTGG GCCATCGCCC 
     7501 TGATAGACGG TTTTTCGCCC TTTGACGTTG GAGTCCACGT TCTTTAATAG TGGACTCTTG 
     7561 TTCCAAACTG GAACAACACT CAACCCTATC TCGGTCTATT CTTTTGATTT ATAAGGGATT 
     7621 TTGCCGATTT CGGCCTATTG GTTAAAAAAT GAGCTGATTT AACAAAAATT TAACGCGAAT 
     7681 TTT
//