LOCUS       RR213892                7953 bp    DNA     circular  SYN 
DEFINITION  Cntn1 (Myc-DDK-tagged ORF) - Rat contactin 1 (Cntn1), (10 ug)
ACCESSION   NM_057118
VERSION     NM_057118.2
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    4108..4199 
                     /label="Myc-DDK"  
     misc_feature    4229..4814 
                     /label="PolyA signal"  
     misc_feature    4286..4305 
                     /label="Restriction Enzyme"  
     misc_feature    5090..5762 
                     /label="ColE1"  
     misc_feature    6192..6986 
                     /label="Kan/Neo"  
     misc_feature    7021..7323 
                     /label="SV40 ori"  
     misc_feature    7409..7886 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    4083..4090 
                     /label="Restriction Enzyme"  
     misc_feature    1020..4083 
                     /label="Cntn1"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GAAAACCCCA TTGCTGGTCA GTCATCTCCT GCTAATCTCC TTAACATCCT 
     1081 GTCTAGGAGA GTTTACATGG CACAGAAGAT ATGGTCATGG AGTTTCTGAG GAGGATAAAG 
     1141 GATTTGGACC CATTTTTGAA GAGCAACCAA TCAATACCAT TTACCCAGAA GAGTCACTGG 
     1201 AAGGCAAAGT GTCGCTCAAC TGCCGGGCAC GGGCAAGCCC ATTTCCGGTT TACAAGTGGA 
     1261 GAATGAATAA CGGGGACGTT GACCTGACGA ACGATCGATA CAGCATGGTC GGAGGGAACC 
     1321 TTGTTATCAA TAACCCTGAC AAACAGAAAG ACGCAGGAAT ATACTACTGC TTGGCGTCCA 
     1381 ACAACTACGG GATGGTCAGA AGCACCGAGG CCACCCTGAG CTTTGGGTAT CTTGACCCCT 
     1441 TTCCTCCTGA GGATCGTCCT GAAGTGAAGG TGAAGGAAGG GAAGGGCATG GTGCTTCTCT 
     1501 GCGACCCTCC CTACCACTTC CCAGACGATC TTAGCTACCG CTGGCTTCTG AATGAATTTC 
     1561 CCGTCTTTAT CACAATGGAT AAGCGAAGAT TCGTGTCTCA GACCAACGGT AATCTCTACA 
     1621 TCGCGAACGT TGAGTCTTCT GATAGAGGCA ACTATTCCTG CTTCGTGTCC AGCCCTTCTA 
     1681 TTACAAAGAG CGTGTTCAGC AAATTTATAC CCCTCATTCC GATACCTGAG CGAACAACGA 
     1741 AGCCATATCC TGCTGACATT GTAGTGCAGT TCAAGGACAT ATACACCATG ATGGGCCAAA 
     1801 ATGTGACCTT AGAGTGCTTC GCTCTTGGAA ACCCTGTTCC TGATATTCGG TGGCGGAAGG 
     1861 TGCTAGAACC CATGCCTACC ACAGCTGAGA TCAGCACCTC AGGGGCTGTC CTCAAGATCT 
     1921 TCAACATCCA GCTAGAAGAT GAAGGGCTCT ACGAGTGCGA GGCTGAGAAC ATTAGGGGAA 
     1981 AGGACAAGCA CCAGGCAAGA ATTTATGTTC AAGCATTTCC TGAATGGGTA GAGCATATCA 
     2041 ATGACACTGA AGTGGACATA GGCAGTGACC TCTACTGGCC TTGTGTGGCC ACAGGGAAGC 
     2101 CCATCCCTAC CATCAGGTGG CTGAAAAATG GGTACGCGTA TCACAAAGGG GAACTGAGGC 
     2161 TGTATGACGT GACTTTTGAA AACGCCGGGA TGTACCAGTG CATAGCAGAA AACGCGTATG 
     2221 GGACCATTTA TGCAAATGCT GAACTCAAGA TCCTGGCTTT AGCTCCAACT TTTGAAATGA 
     2281 ATCCCATGAA GAAGAAGATC CTGGCTGCGA AAGGAGGAAG GGTTATCATT GAGTGCAAAC 
     2341 CCAAGGCTGC ACCAAAACCC AAGTTTTCAT GGAGCAAAGG GACAGAGTGG CTTGTCAACA 
     2401 GCAGCAGAAT ACTCATATGG GAAGATGGTA GCTTGGAAAT CAATAATATT ACAAGAAATG 
     2461 ATGGAGGAAT CTATACATGC TTTGCAGAAA ACAACAGAGG GAAAGCTAAT AGCACCGGGA 
     2521 CTCTGGTGAT CACAAATCCC ACACGAATCA TTTTGGCCCC AATCAATGCT GATATCACTG 
     2581 TTGGAGAAAA CGCTACCATG CAGTGTGCTG CCTCCTTTGA TCCTTCCTTG GATCTCACGT 
     2641 TCGTCTGGTC TTTCAATGGC TACGTGATTG ACTTTAACAA GGAAATTACA AATATTCATT 
     2701 ACCAGAGGAA TTTTATGCTC GACGCCAATG GGGAACTGCT CATCCGAAAC GCGCAGCTGA 
     2761 AACACGCAGG ACGGTACACG TGCACAGCGC AGACAATCGT GGACAATTCT TCAGCTTCAG 
     2821 CTGACCTTGT CGTACGAGGC CCCCCAGGCC CTCCAGGTGG CCTGAGAATA GAAGACATCA 
     2881 GAGCTACCTC TGTGGCACTG ACTTGGAGCC GTGGTTCAGA CAATCACAGC CCTATCTCTA 
     2941 AATACACTAT CCAGACCAAG ACGATTCTCT CAGATGACTG GAAAGACGCA AAGACGGATC 
     3001 CCCCAATCAT TGAAGGAAAC ATGGAGTCAG CAAAAGCAGT CGATTTGATC CCATGGATGG 
     3061 AGTACGAATT TCGAGTGGTA GCAACCAATA CTTTGGGGAC TGGAGAGCCC AGCATACCCT 
     3121 CAAACAGGAT CAAAACCGAT GGCGCTGCAC CAAATGTGGC TCCCTCAGAC GTAGGTGGTG 
     3181 GGGGCGGAAC CAACAGAGAG CTGACAATAA CGTGGGCGCC TTTGTCCAGA GAATACCATT 
     3241 ATGGTAACAA TTTTGGTTAC ATAGTGGCAT TCAAGCCATT CGATGGAGAA GAGTGGAAGA 
     3301 AAGTGACCGT TACCAATCCG GATACTGGCC GCTACGTCCA CAAGGACGAA ACCATGACCC 
     3361 CTTCCACCGC GTTTCAAGTC AAAGTGAAGG CATTCAACAA TAAAGGAGAC GGGCCCTACA 
     3421 GCCTCATAGC TGTCATCAAC TCAGCACAAG ACGCTCCCAG TGAAGCCCCC ACAGAAGTAG 
     3481 GGGTCAAAGT GCTATCGTCC TCTGAGATCT CTGTCCATTG GAAGCACGTT CTAGAGAAGA 
     3541 TTGTGGAAAG CTATCAAATT CGGTACTGGG CCGGTCACGA CAAAGAAGCA GCGGCGCACA 
     3601 GAGTTCAAGT CACCAGCCAG GAGTACTCAG CCAGGCTGGA GAACCTTCTG CCGGACACTC 
     3661 AGTACTTCAT AGAGGTGGGA GCCTGTAACA GCGCGGGGTG CGGACCTTCC AGCGACGTGA 
     3721 TCGAGACCTT CACCAGAAAA GCCCCTCCCA GCCAACCACC AAGGATCATC AGTTCCGTGA 
     3781 GGTCTGGCTC CCGATACATA ATCACGTGGG ATCATGTCGT GGCACTATCC AATGAATCTA 
     3841 CAGTGACAGG CTACAAGATA CTCTATAGAC CTGACGGCCA ACATGACGGT AAGCTGTTCT 
     3901 CCACGCACAA GCACTCCATA GAAGTCCCCA TCCCCAGAGA CGGAGAGTAC GTAGTCGAAG 
     3961 TTCGAGCACA CAGTGATGGA GGAGATGGGG TAGTGTCCCA AGTCAAAATT TCAGGTGTGT 
     4021 CCACGCTGTC ATCGGGTCTC CTCAGCTTGC TCCTGCCCTC CCTTGGCTTT CTTGTCTTCT 
     4081 ATTCGGAATT CAGCGGACCG ACGCGTACGC GGCCGCTCGA GCAGAAACTC ATCTCAGAAG 
     4141 AGGATCTGGC AGCAAATGAT ATCCTGGATT ACAAGGATGA CGACGATAAG GTTTAAACGG 
     4201 CCGGCCGCGG TCATAGCTGT TTCCTGAACA GATCCCGGGT GGCATCCCTG TGACCCCTCC 
     4261 CCAGTGCCTC TCCTGGCCCT GGAAGTTGCC ACTCCAGTGC CCACCAGCCT TGTCCTAATA 
     4321 AAATTAAGTT GCATCATTTT GTCTGACTAG GTGTCCTTCT ATAATATTAT GGGGTGGAGG 
     4381 GGGGTGGTAT GGAGCAAGGG GCAAGTTGGG AAGACAACCT GTAGGGCCTG CGGGGTCTAT 
     4441 TGGGAACCAA GCTGGAGTGC AGTGGCACAA TCTTGGCTCA CTGCAATCTC CGCCTCCTGG 
     4501 GTTCAAGCGA TTCTCCTGCC TCAGCCTCCC GAGTTGTTGG GATTCCAGGC ATGCATGACC 
     4561 AGGCTCAGCT AATTTTTGTT TTTTTGGTAG AGACGGGGTT TCACCATATT GGCCAGGCTG 
     4621 GTCTCCAACT CCTAATCTCA GGTGATCTAC CCACCTTGGC CTCCCAAATT GCTGGGATTA 
     4681 CAGGCGTGAA CCACTGCTCC CTTCCCTGTC CTTCTGATTT TAAAATAACT ATACCAGCAG 
     4741 GAGGACGTCC AGACACAGCA TAGGCTACCT GGCCATGCCC AACCGGTGGG ACATTTGAGT 
     4801 TGCTTGCTTG GCACTGTCCT CTCATGCGTT GGGTCCACTC AGTAGATGCC TGTTGAATTG 
     4861 GGTACGCGGC CAGCGGCGAG CGGTATCAGC TCACTCAAAG GCGGTAATAC GGTTATCCAC 
     4921 AGAATCAGGG GATAACGCAG GAAAGAACAT GTGAGCAAAA GGCCAGCAAA AGGCCAGGAA 
     4981 CCGTAAAAAG GCCGCGTTGC TGGCGTTTTT CCATAGGCTC CGCCCCCCTG ACGAGCATCA 
     5041 CAAAAATCGA CGCTCAAGTC AGAGGTGGCG AAACCCGACA GGACTATAAA GATACCAGGC 
     5101 GTTTCCCCCT GGAAGCTCCC TCGTGCGCTC TCCTGTTCCG ACCCTGCCGC TTACCGGATA 
     5161 CCTGTCCGCC TTTCTCCCTT CGGGAAGCGT GGCGCTTTCT CATAGCTCAC GCTGTAGGTA 
     5221 TCTCAGTTCG GTGTAGGTCG TTCGCTCCAA GCTGGGCTGT GTGCACGAAC CCCCCGTTCA 
     5281 GCCCGACCGC TGCGCCTTAT CCGGTAACTA TCGTCTTGAG TCCAACCCGG TAAGACACGA 
     5341 CTTATCGCCA CTGGCAGCAG CCACTGGTAA CAGGATTAGC AGAGCGAGGT ATGTAGGCGG 
     5401 TGCTACAGAG TTCTTGAAGT GGTGGCCTAA CTACGGCTAC ACTAGAAGAA CAGTATTTGG 
     5461 TATCTGCGCT CTGCTGAAGC CAGTTACCTT CGGAAAAAGA GTTGGTAGCT CTTGATCCGG 
     5521 CAAACAAACC ACCGCTGGTA GCGGTGGTTT TTTTGTTTGC AAGCAGCAGA TTACGCGCAG 
     5581 AAAAAAAGGA TCTCAAGAAG ATCCTTTGAT CTTTTCTACG GGGTCTGACG CTCAGTGGAA 
     5641 CGAAAACTCA CGTTAAGGGA TTTTGGTCAT GAGATTATCA AAAAGGATCT TCACCTAGAT 
     5701 CCTTTTAAAT TAAAAATGAA GTTTTAAATC AATCTAAAGT ATATATGAGT AACCTGAGGC 
     5761 TATGGCAGGG CCTGCCGCCC CGACGTTGGC TGCGAGCCCT GGGCCTTCAC CCGAACTTGG 
     5821 GGGGTGGGGT GGGGAAAAGG AAGAAACGCG GGCGTATTGG CCCCAATGGG GTCTCGGTGG 
     5881 GGTATCGACA GAGTGCCAGC CCTGGGACCG AACCCCGCGT TTATGAACAA ACGACCCAAC 
     5941 ACCGTGCGTT TTATTCTGTC TTTTTATTGC CGTCATAGCG CGGGTTCCTT CCGGTATTGT 
     6001 CTCCTTCCGT GTTTCAGTTA GCCTCCCCCT AGGGTGGGCG AAGAACTCCA GCATGAGATC 
     6061 CCCGCGCTGG AGGATCATCC AGCCGGCGTC CCGGAAAACG ATTCCGAAGC CCAACCTTTC 
     6121 ATAGAAGGCG GCGGTGGAAT CGAAATCTCG TGATGGCAGG TTGGGCGTCG CTTGGTCGGT 
     6181 CATTTCGAAC CCCAGAGTCC CGCTCAGAAG AACTCGTCAA GAAGGCGATA GAAGGCGATG 
     6241 CGCTGCGAAT CGGGAGCGGC GATACCGTAA AGCACGAGGA AGCGGTCAGC CCATTCGCCG 
     6301 CCAAGCTCTT CAGCAATATC ACGGGTAGCC AACGCTATGT CCTGATAGCG ATCCGCCACA 
     6361 CCCAGCCGGC CACAGTCGAT GAATCCAGAA AAGCGGCCAT TTTCCACCAT GATATTCGGC 
     6421 AAGCAGGCAT CGCCATGGGT CACGACGAGA TCCTCGCCGT CGGGCATGCT CGCCTTGAGC 
     6481 CTGGCGAACA GTTCGGCTGG CGCGAGCCCC TGATGCTCTT CGTCCAGATC ATCCTGATCG 
     6541 ACAAGACCGG CTTCCATCCG AGTACGTGCT CGCTCGATGC GATGTTTCGC TTGGTGGTCG 
     6601 AATGGGCAGG TAGCCGGATC AAGCGTATGC AGCCGCCGCA TTGCATCAGC CATGATGGAT 
     6661 ACTTTCTCGG CAGGAGCAAG GTGAGATGAC AGGAGATCCT GCCCCGGCAC TTCGCCCAAT 
     6721 AGCAGCCAGT CCCTTCCCGC TTCAGTGACA ACGTCGAGCA CAGCTGCGCA AGGAACGCCC 
     6781 GTCGTGGCCA GCCACGATAG CCGCGCTGCC TCGTCTTGCA GTTCATTCAG GGCACCGGAC 
     6841 AGGTCGGTCT TGACAAAAAG AACCGGGCGC CCCTGCGCTG ACAGCCGGAA CACGGCGGCA 
     6901 TCAGAGCAGC CGATTGTCTG TTGTGCCCAG TCATAGCCGA ATAGCCTCTC CACCCAAGCG 
     6961 GCCGGAGAAC CTGCGTGCAA TCCATCTTGT TCAATCATGC GAAACGATCC TCATCCTGTC 
     7021 TCTTGATCGA TCTTTGCAAA AGCCTAGGCC TCCAAAAAAG CCTCCTCACT ACTTCTGGAA 
     7081 TAGCTCAGAG GCCGAGGCGG CCTCGGCCTC TGCATAAATA AAAAAAATTA GTCAGCCATG 
     7141 GGGCGGAGAA TGGGCGGAAC TGGGCGGAGT TAGGGGCGGG ATGGGCGGAG TTAGGGGCGG 
     7201 GACTATGGTT GCTGACTAAT TGAGATGCAT GCTTTGCATA CTTCTGCCTG CTGGGGAGCC 
     7261 TGGGGACTTT CCACACCTGG TTGCTGACTA ATTGAGATGC ATGCTTTGCA TACTTCTGCC 
     7321 TGCTGGGGAG CCTGGGGACT TTCCACACCC TAACTGACAC ACATTCCACA GCTGGTTCTT 
     7381 TCCGCCTCAG GACTCTTCCT TTTTCAATAT TATTGAAGCA TTTATCAGGG TTATTGTCTC 
     7441 ATGAGCGGAT ACATATTTGA ATGTATTTAG AAAAATAAAC AAATAGGGGT TCCGCGCACA 
     7501 TTTCCCCGAA AAGTGCCACC TGACGCGCCC TGTAGCGGCG CATTAAGCGC GGCGGGTGTG 
     7561 GTGGTTACGC GCAGCGTGAC CGCTACACTT GCCAGCGCCC TAGCGCCCGC TCCTTTCGCT 
     7621 TTCTTCCCTT CCTTTCTCGC CACGTTCGCC GGCTTTCCCC GTCAAGCTCT AAATCGGGGG 
     7681 CTCCCTTTAG GGTTCCGATT TAGTGCTTTA CGGCACCTCG ACCCCAAAAA ACTTGATTAG 
     7741 GGTGATGGTT CACGTAGTGG GCCATCGCCC TGATAGACGG TTTTTCGCCC TTTGACGTTG 
     7801 GAGTCCACGT TCTTTAATAG TGGACTCTTG TTCCAAACTG GAACAACACT CAACCCTATC 
     7861 TCGGTCTATT CTTTTGATTT ATAAGGGATT TTGCCGATTT CGGCCTATTG GTTAAAAAAT 
     7921 GAGCTGATTT AACAAAAATT TAACGCGAAT TTT
//