LOCUS       RR201101                8475 bp    DNA     circular  SYN 
DEFINITION  Atp2b2 (Myc-DDK-tagged ORF) - Rat ATPase, Ca++ transporting, plasma membrane 2 (Atp2b2), (10 ug)
ACCESSION   NM_012508
VERSION     NM_012508.5
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    4632..4723 
                     /label="Myc-DDK"  
     misc_feature    4753..5338 
                     /label="PolyA signal"  
     misc_feature    4810..4829 
                     /label="Restriction Enzyme"  
     misc_feature    5614..6286 
                     /label="ColE1"  
     misc_feature    6716..7510 
                     /label="Kan/Neo"  
     misc_feature    7545..7847 
                     /label="SV40 ori"  
     misc_feature    7933..8410 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    4614..4620 
                     /label="Restriction Enzyme"  
     misc_feature    1020..4614 
                     /label="Atp2b2"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GGGTGATATG ACCAACAGCG ACTTTTACTC CAAAAACCAA AGAAATGAGT 
     1081 CGAGCCATGG GGGCGAGTTC GGGTGCAGCA TGGAGGAGCT GCGCTCCCTC ATGGAGCTGC 
     1141 GGGGCACTGA GGCTGTGGTC AAGATCAAGG AGACATATGG GGACACTGAA TCCATCTGCC 
     1201 GGCGCCTCAA AACCTCTCCT GTTGAAGGTT TGCCAGGCAC CGCTCCAGAC CTGGAAAAGA 
     1261 GGAAACAGAT TTTCGGGCAA AACTTCATAC CTCCAAAGAA ACCAAAAACC TTCCTGCAGC 
     1321 TGGTGTGGGA AGCACTACAG GACGTGACGC TCATCATCCT GGAGATCGCG GCCATCATCT 
     1381 CCCTGGGACT GTCCTTCTAC CACCCACCCG GAGAGAGCAA TGAAGGATGT GCCACGGCCC 
     1441 AAGGTGGGGC AGAAGATGAA GGTGAAGCAG AAGCAGGCTG GATCGAGGGG GCTGCCATCC 
     1501 TGCTGTCAGT CATCTGTGTG GTCCTCGTCA CAGCCTTCAA TGATTGGAGT AAAGAGAAAC 
     1561 AGTTCCGGGG CCTGCAGAGC CGAATTGAGC AGGAACAGAA GTTCACCGTA GTCCGGGCTG 
     1621 GCCAGGTGGT CCAGATCCCT GTGGCCGAGA TTGTGGTTGG GGACATTGCC CAGATCAAAT 
     1681 ACGGTGACCT CCTTCCTGCT GATGGCCTCT TCATCCAGGG CAATGACCTC AAGATCGATG 
     1741 AAAGCTCGCT CACAGGGGAG TCTGACCAGG TGCGCAAGTC TGTGGATAAG GACCCTATGT 
     1801 TGCTGTCAGG AACTCATGTG ATGGAAGGCT CAGGACGGAT GGTGGTGACT GCTGTGGGTG 
     1861 TCAACTCTCA AACTGGCATC ATATTTACCC TGCTTGGGGC TGGTGGTGAA GAGGAAGAGA 
     1921 AGAAAGACAA AAAAGCCAAG CAGCAGGATG GGGCAGCTGC TATGGAAATG CAGCCTCTGA 
     1981 AGAGTGCGGA GGGCGGTGAT GCAGATGACA AGAAGAAAGC CAACATGCAC AAGAAAGAGA 
     2041 AGTCGGTGCT TCAGGGCAAG CTCACCAAGC TGGCTGTGCA GATAGGCAAG GCGGGCCTGG 
     2101 TGATGTCGGC CATCACGGTG ATCATCCTGG TGCTCTACTT CACCGTGGAC ACCTTCGTGG 
     2161 TCAACAAGAA GCCGTGGCTG ACGGAGTGCA CACCGGTCTA CGTGCAGTAC TTCGTCAAGT 
     2221 TCTTCATCAT TGGTGTGACG GTGCTGGTGG TCGCTGTGCC CGAGGGCCTC CCTCTGGCTG 
     2281 TCACCATCTC ACTGGCCTAT TCCGTGAAGA AAATGATGAA GGACAACAAC CTGGTCCGTC 
     2341 ACCTGGACGC CTGTGAGACC ATGGGTAACG CCACAGCCAT CTGCTCAGAC AAGACAGGCA 
     2401 CGCTGACCAC CAACCGCATG ACCGTGGTGC AGGCCTATGT CGGTGATGTC CACTACAAGG 
     2461 AGATCCCTGA TCCCAGCTCC ATCAATGCCA AGACATTGGA GCTGCTGGTC AACGCCATTG 
     2521 CCATCAACAG CGCCTACACC ACCAAGATCC TCCCCCCAGA AAAAGAGGGC GCCCTGCCCC 
     2581 GGCAGGTGGG CAACAAGACA GAGTGTGGCC TGCTGGGCTT CGTGCTGGAC CTGAGGCAGG 
     2641 ATTACGAGCC GGTACGCAGC CAGATGCCAG AGGAAAAGCT GTATAAGGTG TACACCTTCA 
     2701 ACTCTGTGCG CAAGTCCATG AGCACCGTCA TCAAGATGCC TGACGAGAGC TTCCGCATGT 
     2761 ACAGCAAAGG TGCTTCGGAG ATTGTGCTCA AAAAGTGCTG CAAGATTCTC AGTGGGGCAG 
     2821 GGGAGCCCCG TGTCTTCCGG CCCCGTGACA GGGATGAGAT GGTTAAGAAG GTGATCGAGC 
     2881 CCATGGCCTG TGACGGGCTC CGTACCATCT GTGTGGCCTA TCGTGACTTC CCCAGCAGCC 
     2941 CTGAGCCTGA CTGGGACAAT GAGAATGACA TTCTCAATGA ACTCACCTGC ATCTGCGTGG 
     3001 TGGGCATCGA AGACCCAGTG CGACCTGAGG TCCCAGAAGC CATCCGCAAG TGCCAGCGGG 
     3061 CCGGTATCAC AGTCCGCATG GTCACCGGTG ACAACATCAA CACAGCCCGG GCCATCGCCA 
     3121 TCAAGTGTGG CATTATCCAC CCTGGAGAGG ACTTCCTGTG CCTGGAAGGC AAAGAATTCA 
     3181 ATCGGAGGAT CCGCAACGAG AAGGGGGAGA TTGAGCAGGA GCGGATTGAC AAGATCTGGC 
     3241 CAAAGCTGAG GGTGCTGGCT CGATCCTCGC CCACGGATAA GCACACCCTG GTCAAAGGCA 
     3301 TCATCGACAG CACACACACT GAGCAGCGGC AGGTGGTGGC TGTGACAGGG GATGGGACCA 
     3361 ACGATGGGCC TGCTCTCAAG AAGGCAGATG TGGGCTTCGC GATGGGCATC GCAGGCACGG 
     3421 ATGTGGCCAA GGAGGCCTCA GACATCATCC TGACAGACGA CAACTTCAGC AGCATCGTTA 
     3481 AGGCAGTGAT GTGGGGCCGT AATGTCTATG ACAGCATATC CAAATTCCTG CAGTTTCAGC 
     3541 TGACTGTCAA TGTGGTGGCT GTGATCGTGG CCTTCACGGG TGCCTGCATT ACACAGGACT 
     3601 CCCCTCTCAA GGCGGTGCAG ATGCTCTGGG TGAACCTCAT CATGGACACA TTTGCTTCCC 
     3661 TGGCCCTGGC AACAGAGCCA CCCACCGAGA CTCTGCTCCT GAGGAAACCT TACGGCCGCA 
     3721 ACAAGCCGCT CATCTCGAGA ACCATGATGA AGAACATCCT GGGCCACGCC GTCTACCAGC 
     3781 TAACCCTCAT CTTCACCCTG CTCTTCGTGG GTGAGAAGAT GTTCCAGATT GACAGCGGAA 
     3841 GGAACGCCCC GCTGCACTCG CCACCCTCGG AGCACTACAC CATCATCTTC AACACCTTCG 
     3901 TCATGATGCA GCTTTTCAAC GAGATCAACG CCCGTAAGAT CCACGGCGAG CGTAACGTCT 
     3961 TCGACGGAAT CTTCCGGAAC CCCATCTTCT GCACCATCGT TCTCGGCACT TTTGCCATCC 
     4021 AGATAGTGAT TGTGCAGTTC GGCGGCAAAC CCTTCAGCTG CTCCCCACTC CAGCTGGACC 
     4081 AGTGGATGTG GTGCATCTTC ATAGGCCTGG GAGAGCTCGT CTGGGGCCAG GTCATTGCCA 
     4141 CCATCCCTAC CAGCAGGCTC AAGTTTCTGA AAGAGGCAGG GCGGCTAACA CAGAAGGAGG 
     4201 AGATCCCCGA GGAAGAGTTA AATGAGGATG TGGAAGAGAT AGACCACGCA GAGCGGGAGC 
     4261 TTCGCCGAGG CCAGATCCTA TGGTTCCGGG GCCTGAATCG GATCCAGACA CAGATCCGCG 
     4321 TCGTGAAGGC GTTCCGTAGC TCTCTCTATG AAGGGTTAGA AAAACCCGAG TCTCGAACCT 
     4381 CCATCCATAA CTTCATGGCT CATCCTGAAT TCCGGATCGA AGATTCCCAG CCCCACATCC 
     4441 CCCTCATCGA TGACACCGAC CTGGAAGAAG ATGCCGCGCT CAAGCAGAAC TCGAGCCCGC 
     4501 CGTCCTCGCT CAACAAGAAC AATAGCGCCA TCGACAGCGG GATCAACCTG ACGACCGACA 
     4561 CGAGCAAATC AGCTACCTCT TCAAGTCCAG GGAGCCCCAT CCACAGCCTG GAGACGTCGC 
     4621 TTACGCGTAC GCGGCCGCTC GAGCAGAAAC TCATCTCAGA AGAGGATCTG GCAGCAAATG 
     4681 ATATCCTGGA TTACAAGGAT GACGACGATA AGGTTTAAAC GGCCGGCCGC GGTCATAGCT 
     4741 GTTTCCTGAA CAGATCCCGG GTGGCATCCC TGTGACCCCT CCCCAGTGCC TCTCCTGGCC 
     4801 CTGGAAGTTG CCACTCCAGT GCCCACCAGC CTTGTCCTAA TAAAATTAAG TTGCATCATT 
     4861 TTGTCTGACT AGGTGTCCTT CTATAATATT ATGGGGTGGA GGGGGGTGGT ATGGAGCAAG 
     4921 GGGCAAGTTG GGAAGACAAC CTGTAGGGCC TGCGGGGTCT ATTGGGAACC AAGCTGGAGT 
     4981 GCAGTGGCAC AATCTTGGCT CACTGCAATC TCCGCCTCCT GGGTTCAAGC GATTCTCCTG 
     5041 CCTCAGCCTC CCGAGTTGTT GGGATTCCAG GCATGCATGA CCAGGCTCAG CTAATTTTTG 
     5101 TTTTTTTGGT AGAGACGGGG TTTCACCATA TTGGCCAGGC TGGTCTCCAA CTCCTAATCT 
     5161 CAGGTGATCT ACCCACCTTG GCCTCCCAAA TTGCTGGGAT TACAGGCGTG AACCACTGCT 
     5221 CCCTTCCCTG TCCTTCTGAT TTTAAAATAA CTATACCAGC AGGAGGACGT CCAGACACAG 
     5281 CATAGGCTAC CTGGCCATGC CCAACCGGTG GGACATTTGA GTTGCTTGCT TGGCACTGTC 
     5341 CTCTCATGCG TTGGGTCCAC TCAGTAGATG CCTGTTGAAT TGGGTACGCG GCCAGCGGCG 
     5401 AGCGGTATCA GCTCACTCAA AGGCGGTAAT ACGGTTATCC ACAGAATCAG GGGATAACGC 
     5461 AGGAAAGAAC ATGTGAGCAA AAGGCCAGCA AAAGGCCAGG AACCGTAAAA AGGCCGCGTT 
     5521 GCTGGCGTTT TTCCATAGGC TCCGCCCCCC TGACGAGCAT CACAAAAATC GACGCTCAAG 
     5581 TCAGAGGTGG CGAAACCCGA CAGGACTATA AAGATACCAG GCGTTTCCCC CTGGAAGCTC 
     5641 CCTCGTGCGC TCTCCTGTTC CGACCCTGCC GCTTACCGGA TACCTGTCCG CCTTTCTCCC 
     5701 TTCGGGAAGC GTGGCGCTTT CTCATAGCTC ACGCTGTAGG TATCTCAGTT CGGTGTAGGT 
     5761 CGTTCGCTCC AAGCTGGGCT GTGTGCACGA ACCCCCCGTT CAGCCCGACC GCTGCGCCTT 
     5821 ATCCGGTAAC TATCGTCTTG AGTCCAACCC GGTAAGACAC GACTTATCGC CACTGGCAGC 
     5881 AGCCACTGGT AACAGGATTA GCAGAGCGAG GTATGTAGGC GGTGCTACAG AGTTCTTGAA 
     5941 GTGGTGGCCT AACTACGGCT ACACTAGAAG AACAGTATTT GGTATCTGCG CTCTGCTGAA 
     6001 GCCAGTTACC TTCGGAAAAA GAGTTGGTAG CTCTTGATCC GGCAAACAAA CCACCGCTGG 
     6061 TAGCGGTGGT TTTTTTGTTT GCAAGCAGCA GATTACGCGC AGAAAAAAAG GATCTCAAGA 
     6121 AGATCCTTTG ATCTTTTCTA CGGGGTCTGA CGCTCAGTGG AACGAAAACT CACGTTAAGG 
     6181 GATTTTGGTC ATGAGATTAT CAAAAAGGAT CTTCACCTAG ATCCTTTTAA ATTAAAAATG 
     6241 AAGTTTTAAA TCAATCTAAA GTATATATGA GTAACCTGAG GCTATGGCAG GGCCTGCCGC 
     6301 CCCGACGTTG GCTGCGAGCC CTGGGCCTTC ACCCGAACTT GGGGGGTGGG GTGGGGAAAA 
     6361 GGAAGAAACG CGGGCGTATT GGCCCCAATG GGGTCTCGGT GGGGTATCGA CAGAGTGCCA 
     6421 GCCCTGGGAC CGAACCCCGC GTTTATGAAC AAACGACCCA ACACCGTGCG TTTTATTCTG 
     6481 TCTTTTTATT GCCGTCATAG CGCGGGTTCC TTCCGGTATT GTCTCCTTCC GTGTTTCAGT 
     6541 TAGCCTCCCC CTAGGGTGGG CGAAGAACTC CAGCATGAGA TCCCCGCGCT GGAGGATCAT 
     6601 CCAGCCGGCG TCCCGGAAAA CGATTCCGAA GCCCAACCTT TCATAGAAGG CGGCGGTGGA 
     6661 ATCGAAATCT CGTGATGGCA GGTTGGGCGT CGCTTGGTCG GTCATTTCGA ACCCCAGAGT 
     6721 CCCGCTCAGA AGAACTCGTC AAGAAGGCGA TAGAAGGCGA TGCGCTGCGA ATCGGGAGCG 
     6781 GCGATACCGT AAAGCACGAG GAAGCGGTCA GCCCATTCGC CGCCAAGCTC TTCAGCAATA 
     6841 TCACGGGTAG CCAACGCTAT GTCCTGATAG CGATCCGCCA CACCCAGCCG GCCACAGTCG 
     6901 ATGAATCCAG AAAAGCGGCC ATTTTCCACC ATGATATTCG GCAAGCAGGC ATCGCCATGG 
     6961 GTCACGACGA GATCCTCGCC GTCGGGCATG CTCGCCTTGA GCCTGGCGAA CAGTTCGGCT 
     7021 GGCGCGAGCC CCTGATGCTC TTCGTCCAGA TCATCCTGAT CGACAAGACC GGCTTCCATC 
     7081 CGAGTACGTG CTCGCTCGAT GCGATGTTTC GCTTGGTGGT CGAATGGGCA GGTAGCCGGA 
     7141 TCAAGCGTAT GCAGCCGCCG CATTGCATCA GCCATGATGG ATACTTTCTC GGCAGGAGCA 
     7201 AGGTGAGATG ACAGGAGATC CTGCCCCGGC ACTTCGCCCA ATAGCAGCCA GTCCCTTCCC 
     7261 GCTTCAGTGA CAACGTCGAG CACAGCTGCG CAAGGAACGC CCGTCGTGGC CAGCCACGAT 
     7321 AGCCGCGCTG CCTCGTCTTG CAGTTCATTC AGGGCACCGG ACAGGTCGGT CTTGACAAAA 
     7381 AGAACCGGGC GCCCCTGCGC TGACAGCCGG AACACGGCGG CATCAGAGCA GCCGATTGTC 
     7441 TGTTGTGCCC AGTCATAGCC GAATAGCCTC TCCACCCAAG CGGCCGGAGA ACCTGCGTGC 
     7501 AATCCATCTT GTTCAATCAT GCGAAACGAT CCTCATCCTG TCTCTTGATC GATCTTTGCA 
     7561 AAAGCCTAGG CCTCCAAAAA AGCCTCCTCA CTACTTCTGG AATAGCTCAG AGGCCGAGGC 
     7621 GGCCTCGGCC TCTGCATAAA TAAAAAAAAT TAGTCAGCCA TGGGGCGGAG AATGGGCGGA 
     7681 ACTGGGCGGA GTTAGGGGCG GGATGGGCGG AGTTAGGGGC GGGACTATGG TTGCTGACTA 
     7741 ATTGAGATGC ATGCTTTGCA TACTTCTGCC TGCTGGGGAG CCTGGGGACT TTCCACACCT 
     7801 GGTTGCTGAC TAATTGAGAT GCATGCTTTG CATACTTCTG CCTGCTGGGG AGCCTGGGGA 
     7861 CTTTCCACAC CCTAACTGAC ACACATTCCA CAGCTGGTTC TTTCCGCCTC AGGACTCTTC 
     7921 CTTTTTCAAT ATTATTGAAG CATTTATCAG GGTTATTGTC TCATGAGCGG ATACATATTT 
     7981 GAATGTATTT AGAAAAATAA ACAAATAGGG GTTCCGCGCA CATTTCCCCG AAAAGTGCCA 
     8041 CCTGACGCGC CCTGTAGCGG CGCATTAAGC GCGGCGGGTG TGGTGGTTAC GCGCAGCGTG 
     8101 ACCGCTACAC TTGCCAGCGC CCTAGCGCCC GCTCCTTTCG CTTTCTTCCC TTCCTTTCTC 
     8161 GCCACGTTCG CCGGCTTTCC CCGTCAAGCT CTAAATCGGG GGCTCCCTTT AGGGTTCCGA 
     8221 TTTAGTGCTT TACGGCACCT CGACCCCAAA AAACTTGATT AGGGTGATGG TTCACGTAGT 
     8281 GGGCCATCGC CCTGATAGAC GGTTTTTCGC CCTTTGACGT TGGAGTCCAC GTTCTTTAAT 
     8341 AGTGGACTCT TGTTCCAAAC TGGAACAACA CTCAACCCTA TCTCGGTCTA TTCTTTTGAT 
     8401 TTATAAGGGA TTTTGCCGAT TTCGGCCTAT TGGTTAAAAA ATGAGCTGAT TTAACAAAAA 
     8461 TTTAACGCGA ATTTT
//