LOCUS       RG226266                9371 bp    DNA     circular  SYN 
DEFINITION  C6 (tGFP-tagged) - Human complement component 6 (C6), transcript variant 2
ACCESSION   NM_001115131
VERSION     null
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    3850..4554 
                     /label="GFP"  
     misc_feature    4601..5186 
                     /label="PolyA signal"  
     misc_feature    7278..7923 
                     /label="ColE1"  
     misc_feature    5640..6434 
                     /label="Neo"  
     misc_feature    5271..5573 
                     /label="SV40 ori"  
     misc_feature    7938..8798 
                     /label="Amp"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    3822..3829 
                     /label="Restriction Enzyme"  
     misc_feature    1020..3822 
                     /label="C6"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GGCCAGACGC TCTGTCTTGT ACTTCATCCT GCTGAATGCT CTGATCAACA 
     1081 AGGGCCAAGC CTGCTTCTGT GATCACTATG CATGGACTCA GTGGACCAGC TGCTCAAAAA 
     1141 CTTGCAATTC TGGAACCCAG AGCAGACACA GACAAATAGT AGTAGATAAG TACTACCAGG 
     1201 AAAACTTTTG TGAACAGATT TGCAGCAAGC AGGAGACTAG AGAATGTAAC TGGCAAAGAT 
     1261 GCCCCATCAA CTGCCTCCTG GGAGATTTTG GACCATGGTC AGACTGTGAC CCTTGTATTG 
     1321 AAAAACAGTC TAAAGTTAGA TCTGTCTTGC GTCCCAGTCA GTTTGGGGGA CAGCCATGCA 
     1381 CTGCGCCTCT GGTAGCCTTT CAACCATGCA TTCCATCTAA GCTCTGCAAA ATTGAAGAGG 
     1441 CTGACTGCAA GAATAAATTT CGCTGTGACA GTGGCCGCTG CATTGCCAGA AAGTTAGAAT 
     1501 GCAATGGAGA AAATGACTGT GGAGACAATT CAGATGAAAG GGACTGTGGG AGGACAAAGG 
     1561 CAGTATGCAC ACGGAAGTAT AATCCCATCC CTAGTGTACA GTTGATGGGC AATGGGTTTC 
     1621 ATTTTCTGGC AGGAGAGCCC AGAGGAGAAG TCCTTGATAA CTCTTTCACT GGAGGAATAT 
     1681 GTAAAACTGT CAAAAGCAGT AGGACAAGTA ATCCATACCG TGTTCCGGCC AATCTGGAAA 
     1741 ATGTCGGCTT TGAGGTACAA ACTGCAGAAG ATGACTTGAA AACAGATTTC TACAAGGATT 
     1801 TAACTTCTCT TGGACACAAT GAAAATCAAC AAGGCTCATT CTCAAGTCAG GGGGGGAGCT 
     1861 CTTTCAGTGT ACCAATTTTT TATTCCTCAA AGAGAAGTGA AAATATCAAC CATAATTCTG 
     1921 CCTTCAAACA AGCCATTCAA GCCTCTCACA AAAAGGATTC TAGTTTTATT AGGATCCATA 
     1981 AAGTGATGAA AGTCTTAAAC TTCACAACGA AAGCTAAAGA TCTGCACCTT TCTGATGTCT 
     2041 TTTTGAAAGC ACTTAACCAT CTGCCTCTAG AATACAACTC TGCTTTGTAC AGCCGAATAT 
     2101 TCGATGACTT TGGGACTCAT TACTTCACCT CTGGCTCCCT GGGAGGCGTG TATGACCTTC 
     2161 TCTATCAGTT TAGCAGTGAG GAACTAAAGA ACTCAGGTTT AACCGAGGAA GAAGCCAAAC 
     2221 ACTGTGTCAG GATTGAAACA AAGAAACGCG TTTTATTTGC TAAGAAAACA AAAGTGGAAC 
     2281 ATAGGTGCAC CACCAACAAG CTGTCAGAGA AACATGAAGG TTCATTTATA CAGGGAGCAG 
     2341 AGAAATCCAT ATCCCTGATT CGAGGTGGAA GGAGTGAATA TGGAGCAGCT TTGGCATGGG 
     2401 AGAAAGGGAG CTCTGGTCTG GAGGAGAAGA CATTTTCTGA GTGGTTAGAA TCAGTGAAGG 
     2461 AAAATCCTGC TGTGATTGAC TTTGAGCTTG CCCCCATCGT GGACTTGGTA AGAAACATCC 
     2521 CCTGTGCAGT GACAAAACGG AACAACCTCA GGAAAGCTTT GCAAGAGTAT GCAGCCAAGT 
     2581 TCGATCCTTG CCAGTGTGCT CCATGCCCTA ATAATGGCCG ACCCACCCTC TCAGGGACTG 
     2641 AATGTCTGTG TGTGTGTCAG AGTGGCACCT ATGGTGAGAA CTGTGAGAAA CAGTCTCCAG 
     2701 ATTATAAATC CAATGCAGTA GACGGACAGT GGGGTTGTTG GTCTTCCTGG AGTACCTGTG 
     2761 ATGCTACTTA TAAGAGATCG AGAACCCGAG AATGCAATAA TCCTGCCCCC CAACGAGGAG 
     2821 GGAAACGCTG TGAGGGGGAG AAGCGACAAG AGGAAGACTG CACATTTTCA ATCATGGAAA 
     2881 ACAATGGACA ACCATGTATC AATGATGATG AAGAAATGAA AGAGGTCGAT CTTCCTGAGA 
     2941 TAGAAGCAGA TTCCGGGTGT CCTCAGCCAG TTCCTCCAGA AAATGGATTT ATCCGGAATG 
     3001 AAAAGCAACT ATACTTGGTT GGAGAAGATG TTGAAATTTC ATGCCTTACT GGCTTTGAAA 
     3061 CTGTTGGATA CCAGTACTTC AGATGCTTAC CAGACGGGAC CTGGAGACAA GGGGATGTGG 
     3121 AATGCCAACG GACGGAGTGC ATCAAGCCAG TTGTGCAGGA AGTCCTGACA ATTACACCAT 
     3181 TTCAGAGATT GTATAGAATT GGTGAATCCA TTGAGCTAAC TTGCCCCAAA GGCTTTGTTG 
     3241 TTGCTGGGCC ATCAAGGTAC ACATGCCAGG GGAATTCCTG GACACCACCC ATTTCAAACT 
     3301 CTCTCACCTG TGAAAAAGAT ACTCTAACAA AATTAAAAGG CCATTGTCAG CTGGGACAGA 
     3361 AACAATCAGG ATCTGAATGC ATTTGTATGT CTCCAGAAGA AGACTGTAGC CATCATTCAG 
     3421 AAGATCTCTG TGTGTTTGAC ACAGACTCCA ACGATTACTT TACTTCACCC GCTTGTAAGT 
     3481 TTTTGGCTGA GAAATGTTTA AATAATCAGC AACTCCATTT TCTACATATT GGTTCCTGCC 
     3541 AAGACGGCCG CCAGTTAGAA TGGGGTCTTG AAAGGACAAG ACTTTCATCC AACAGCACAA 
     3601 AGAAAGAATC CTGTGGCTAT GACACCTGCT ATGACTGGGA AAAATGTTCA GCCTCCACTT 
     3661 CCAAATGTGT CTGCCTATTG CCCCCACAGT GCTTCAAGGG TGGAAACCAA CTCTACTGTG 
     3721 TCAAAATGGG ATCATCAACA AGTGAGAAAA CATTGAACAT CTGTGAAGTG GGAACTATAA 
     3781 GATGTGCAAA CAGGAAGATG GAAATACTGC ATCCTGGAAA GTGTTTGGCC AGCGGACCGA 
     3841 CGCGTACGCG GCCGCTCGAG ATGGAGAGCG ACGAGAGCGG CCTGCCCGCC ATGGAGATCG 
     3901 AGTGCCGCAT CACCGGCACC CTGAACGGCG TGGAGTTCGA GCTGGTGGGC GGCGGAGAGG 
     3961 GCACCCCCGA GCAGGGCCGC ATGACCAACA AGATGAAGAG CACCAAAGGC GCCCTGACCT 
     4021 TCAGCCCCTA CCTGCTGAGC CACGTGATGG GCTACGGCTT CTACCACTTC GGCACCTACC 
     4081 CCAGCGGCTA CGAGAACCCC TTCCTGCACG CCATCAACAA CGGCGGCTAC ACCAACACCC 
     4141 GCATCGAGAA GTACGAGGAC GGCGGCGTGC TGCACGTGAG CTTCAGCTAC CGCTACGAGG 
     4201 CCGGCCGCGT GATCGGCGAC TTCAAGGTGA TGGGCACCGG CTTCCCCGAG GACAGCGTGA 
     4261 TCTTCACCGA CAAGATCATC CGCAGCAACG CCACCGTGGA GCACCTGCAC CCCATGGGCG 
     4321 ATAACGATCT GGATGGCAGC TTCACCCGCA CCTTCAGCCT GCGCGACGGC GGCTACTACA 
     4381 GCTCCGTGGT GGACAGCCAC ATGCACTTCA AGAGCGCCAT CCACCCCAGC ATCCTGCAGA 
     4441 ACGGGGGCCC CATGTTCGCC TTCCGCCGCG TGGAGGAGGA TCACAGCAAC ACCGAGCTGG 
     4501 GCATCGTGGA GTACCAGCAC GCCTTCAAGA CCCCGGATGC AGATGCCGGT GAAGAAAGAG 
     4561 TTTAAACGGC CGGCCGCGGT CATAGCTGTT TCCTGAACAG ATCCCGGGTG GCATCCCTGT 
     4621 GACCCCTCCC CAGTGCCTCT CCTGGCCCTG GAAGTTGCCA CTCCAGTGCC CACCAGCCTT 
     4681 GTCCTAATAA AATTAAGTTG CATCATTTTG TCTGACTAGG TGTCCTTCTA TAATATTATG 
     4741 GGGTGGAGGG GGGTGGTATG GAGCAAGGGG CAAGTTGGGA AGACAACCTG TAGGGCCTGC 
     4801 GGGGTCTATT GGGAACCAAG CTGGAGTGCA GTGGCACAAT CTTGGCTCAC TGCAATCTCC 
     4861 GCCTCCTGGG TTCAAGCGAT TCTCCTGCCT CAGCCTCCCG AGTTGTTGGG ATTCCAGGCA 
     4921 TGCATGACCA GGCTCAGCTA ATTTTTGTTT TTTTGGTAGA GACGGGGTTT CACCATATTG 
     4981 GCCAGGCTGG TCTCCAACTC CTAATCTCAG GTGATCTACC CACCTTGGCC TCCCAAATTG 
     5041 CTGGGATTAC AGGCGTGAAC CACTGCTCCC TTCCCTGTCC TTCTGATTTT AAAATAACTA 
     5101 TACCAGCAGG AGGACGTCCA GACACAGCAT AGGCTACCTG GCCATGCCCA ACCGGTGGGA 
     5161 CATTTGAGTT GCTTGCTTGG CACTGTCCTC TCATGCGTTG GGTCCACTCA GTAGATGCCT 
     5221 GTTGAATTGG GTACGCGGCC AGCTTGGCTG TGGAATGTGT GTCAGTTAGG GTGTGGAAAG 
     5281 TCCCCAGGCT CCCCAGCAGG CAGAAGTATG CAAAGCATGC ATCTCAATTA GTCAGCAACC 
     5341 AGGTGTGGAA AGTCCCCAGG CTCCCCAGCA GGCAGAAGTA TGCAAAGCAT GCATCTCAAT 
     5401 TAGTCAGCAA CCATAGTCCC GCCCCTAACT CCGCCCATCC CGCCCCTAAC TCCGCCCAGT 
     5461 TCCGCCCATT CTCCGCCCCA TGGCTGACTA ATTTTTTTTA TTTATGCAGA GGCCGAGGCC 
     5521 GCCTCGGCCT CTGAGCTATT CCAGAAGTAG TGAGGAGGCT TTTTTGGAGG CCTAGGCTTT 
     5581 TGCAAAAAGC TCCCGGGAGC TTGTATATCC ATTTTCGGAT CTGATCAAGA GACAGGATGA 
     5641 GGATCGTTTC GCATGATTGA ACAAGATGGA TTGCACGCAG GTTCTCCGGC CGCTTGGGTG 
     5701 GAGAGGCTAT TCGGCTATGA CTGGGCACAA CAGACAATCG GCTGCTCTGA TGCCGCCGTG 
     5761 TTCCGGCTGT CAGCGCAGGG GCGCCCGGTT CTTTTTGTCA AGACCGACCT GTCCGGTGCC 
     5821 CTGAATGAAC TGCAGGACGA GGCAGCGCGG CTATCGTGGC TGGCCACGAC GGGCGTTCCT 
     5881 TGCGCAGCTG TGCTCGACGT TGTCACTGAA GCGGGAAGGG ACTGGCTGCT ATTGGGCGAA 
     5941 GTGCCGGGGC AGGATCTCCT GTCATCTCAC CTTGCTCCTG CCGAGAAAGT ATCCATCATG 
     6001 GCTGATGCAA TGCGGCGGCT GCATACGCTT GATCCGGCTA CCTGCCCATT CGACCACCAA 
     6061 GCGAAACATC GCATCGAGCG AGCACGTACT CGGATGGAAG CCGGTCTTGT CGATCAGGAT 
     6121 GATCTGGACG AAGAGCATCA GGGGCTCGCG CCAGCCGAAC TGTTCGCCAG GCTCAAGGCG 
     6181 CGCATGCCCG ACGGCGAGGA TCTCGTCGTG ACCCATGGCG ATGCCTGCTT GCCGAATATC 
     6241 ATGGTGGAAA ATGGCCGCTT TTCTGGATTC ATCGACTGTG GCCGGCTGGG TGTGGCCGAC 
     6301 CGCTATCAGG ACATAGCGTT GGCTACCCGT GATATTGCTG AAGAGCTTGG CGGCGAATGG 
     6361 GCTGACCGCT TCCTCGTGCT TTACGGTATC GCCGCTCCCG ATTCGCAGCG CATCGCCTTC 
     6421 TATCGCCTTC TTGACGAGTT CTTCTGAGCG GGACTCTGGG GTTCGAAATG ACCGACCAAG 
     6481 CGACGCCCAA CCTGCCATCA CGAGATTTCG ATTCCACCGC CGCCTTCTAT GAAAGGTTGG 
     6541 GCTTCGGAAT CGTTTTCCGG GACGCCGGCT GGATGATCCT CCAGCGCGGG GATCTCATGC 
     6601 TGGAGTTCTT CGCCCACCCC AACTTGTTTA TTGCAGCTTA TAATGGTTAC AAATAAAGCA 
     6661 ATAGCATCAC AAATTTCACA AATAAAGCAT TTTTTTCACT GCATTCTAGT TGTGGTTTGT 
     6721 CCAAACTCAT CAATGTATCT TATCATGTCT GTATACCGTC GACCTCTAGC TAGAGCTTGG 
     6781 CGTAATCATG GTCATAGCTG TTTCCTGTGT GAAATTGTTA TCCGCTCACA ATTCCACACA 
     6841 ACATACGAGC CGGAAGCATA AAGTGTAAAG CCTGGGGTGC CTAATGAGTG AGCTAACTCA 
     6901 CATTAATTGC GTTGCGCTCA CTGCCCGCTT TCCAGTCGGG AAACCTGTCG TGCCAGCTGC 
     6961 ATTAATGAAT CGGCCAACGC GCGGGGAGAG GCGGTTTGCG TATTGGGCGC TCTTCCGCTT 
     7021 CCTCGCTCAC TGACTCGCTG CGCTCGGTCG TTCGGCTGCG GCGAGCGGTA TCAGCTCACT 
     7081 CAAAGGCGGT AATACGGTTA TCCACAGAAT CAGGGGATAA CGCAGGAAAG AACATGTGAG 
     7141 CAAAAGGCCA GCAAAAGGCC AGGAACCGTA AAAAGGCCGC GTTGCTGGCG TTTTTCCATA 
     7201 GGCTCCGCCC CCCTGACGAG CATCACAAAA ATCGACGCTC AAGTCAGAGG TGGCGAAACC 
     7261 CGACAGGACT ATAAAGATAC CAGGCGTTTC CCCCTGGAAG CTCCCTCGTG CGCTCTCCTG 
     7321 TTCCGACCCT GCCGCTTACC GGATACCTGT CCGCCTTTCT CCCTTCGGGA AGCGTGGCGC 
     7381 TTTCTCATAG CTCACGCTGT AGGTATCTCA GTTCGGTGTA GGTCGTTCGC TCCAAGCTGG 
     7441 GCTGTGTGCA CGAACCCCCC GTTCAGCCCG ACCGCTGCGC CTTATCCGGT AACTATCGTC 
     7501 TTGAGTCCAA CCCGGTAAGA CACGACTTAT CGCCACTGGC AGCAGCCACT GGTAACAGGA 
     7561 TTAGCAGAGC GAGGTATGTA GGCGGTGCTA CAGAGTTCTT GAAGTGGTGG CCTAACTACG 
     7621 GCTACACTAG AAGAACAGTA TTTGGTATCT GCGCTCTGCT GAAGCCAGTT ACCTTCGGAA 
     7681 AAAGAGTTGG TAGCTCTTGA TCCGGCAAAC AAACCACCGC TGGTAGCGGT TTTTTTGTTT 
     7741 GCAAGCAGCA GATTACGCGC AGAAAAAAAG GATCTCAAGA AGATCCTTTG ATCTTTTCTA 
     7801 CGGGGTCTGA CGCTCAGTGG AACGAAAACT CACGTTAAGG GATTTTGGTC ATGAGATTAT 
     7861 CAAAAAGGAT CTTCACCTAG ATCCTTTTAA ATTAAAAATG AAGTTTTAAA TCAATCTAAA 
     7921 GTATATATGA GTAAACTTGG TCTGACAGTT ACCAATGCTT AATCAGTGAG GCACCTATCT 
     7981 CAGCGATCTG TCTATTTCGT TCATCCATAG TTGCCTGACT CCCCGTCGTG TAGATAACTA 
     8041 CGATACGGGA GGGCTTACCA TCTGGCCCCA GTGCTGCAAT GATACCGCGA GACCCACGCT 
     8101 CACCGGCTCC AGATTTATCA GCAATAAACC AGCCAGCCGG AAGGGCCGAG CGCAGAAGTG 
     8161 GTCCTGCAAC TTTATCCGCC TCCATCCAGT CTATTAATTG TTGCCGGGAA GCTAGAGTAA 
     8221 GTAGTTCGCC AGTTAATAGT TTGCGCAACG TTGTTGCCAT TGCTACAGGC ATCGTGGTGT 
     8281 CACGCTCGTC GTTTGGTATG GCTTCATTCA GCTCCGGTTC CCAACGATCA AGGCGAGTTA 
     8341 CATGATCCCC CATGTTGTGC AAAAAAGCGG TTAGCTCCTT CGGTCCTCCG ATCGTTGTCA 
     8401 GAAGTAAGTT GGCCGCAGTG TTATCACTCA TGGTTATGGC AGCACTGCAT AATTCTCTTA 
     8461 CTGTCATGCC ATCCGTAAGA TGCTTTTCTG TGACTGGTGA GTACTCAACC AAGTCATTCT 
     8521 GAGAATAGTG TATGCGGCGA CCGAGTTGCT CTTGCCCGGC GTCAATACGG GATAATACCG 
     8581 CGCCACATAG CAGAACTTTA AAAGTGCTCA TCATTGGAAA ACGTTCTTCG GGGCGAAAAC 
     8641 TCTCAAGGAT CTTACCGCTG TTGAGATCCA GTTCGATGTA ACCCACTCGT GCACCCAACT 
     8701 GATCTTCAGC ATCTTTTACT TTCACCAGCG TTTCTGGGTG AGCAAAAACA GGAAGGCAAA 
     8761 ATGCCGCAAA AAAGGGAATA AGGGCGACAC GGAAATGTTG AATACTCATA CTCTTCCTTT 
     8821 TTCAATATTA TTGAAGCATT TATCAGGGTT ATTGTCTCAT GAGCGGATAC ATATTTGAAT 
     8881 GTATTTAGAA AAATAAACAA ATAGGGGTTC CGCGCACATT TCCCCGAAAA GTGCCACCTG 
     8941 ACGCGCCCTG TAGCGGCGCA TTAAGCGCGG CGGGTGTGGT GGTTACGCGC AGCGTGACCG 
     9001 CTACACTTGC CAGCGCCCTA GCGCCCGCTC CTTTCGCTTT CTTCCCTTCC TTTCTCGCCA 
     9061 CGTTCGCCGG CTTTCCCCGT CAAGCTCTAA ATCGGGGGCT CCCTTTAGGG TTCCGATTTA 
     9121 GTGCTTTACG GCACCTCGAC CCCAAAAAAC TTGATTAGGG TGATGGTTCA CGTAGTGGGC 
     9181 CATCGCCCTG ATAGACGGTT TTTCGCCCTT TGACGTTGGA GTCCACGTTC TTTAATAGTG 
     9241 GACTCTTGTT CCAAACTGGA ACAACACTCA ACCCTATCTC GGTCTATTCT TTTGATTTAT 
     9301 AAGGGATTTT GCCGATTTCG GCCTATTGGT TAAAAAATGA GCTGATTTAA CAAAAATTTA 
     9361 ACGCGAATTT T
//