LOCUS       RC218385                8652 bp    DNA     circular  SYN 
DEFINITION  IRS4 (Myc-DDK-tagged)-Human insulin receptor substrate 4 (IRS4)
ACCESSION   NM_003604
VERSION     NM_003604.1
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    4809..4900 
                     /label="Myc-DDK"  
     misc_feature    4930..5515 
                     /label="PolyA signal"  
     misc_feature    4987..5006 
                     /label="Restriction Enzyme"  
     misc_feature    5791..6463 
                     /label="ColE1"  
     misc_feature    6893..7687 
                     /label="Kan/Neo"  
     misc_feature    7722..8024 
                     /label="SV40 ori"  
     misc_feature    8110..8587 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    4791..4797 
                     /label="Restriction Enzyme"  
     misc_feature    1020..4791 
                     /label="IRS4"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GGCGAGTTGC TCCTTCACTC GCGACCAAGC GACAAGAAGA CTAAGAGGTG 
     1081 CAGCAGCGGC GGCAGCGGCA GCTCTAGCAG CAGTGGTGAC CACCCCGCTT CTTTCCTCGG 
     1141 GAACCCCGAC CGCACTCATT GGGACCGGGT CGTCTTGTCC GGGAGCCATG TGGCTCTCCA 
     1201 CGGCCACTGG CTCCCGGTCA GACTCCGAGT CCGAAGAGGA GGACCTGCCC GTCGGGGAGG 
     1261 AAGTCTGCAA ACGCGGCTAC CTGCGGAAAC AGAAGCATGG GCACAGGCGC TACTTCGTGC 
     1321 TCAAACTCGA GACTGCTGAC GCCCCAGCTC GGCTGGAATA CTACGAAAAT GCCAGGAAGT 
     1381 TCCGGCACAG TGTCCGCGCC GCGGCGGCTG CAGCAGCGGC GGCCGCCTCT GGCGCCGCGA 
     1441 TCCCCCCGCT CATTCCACCG CGGCGCGTGA TCACCCTATA CCAGTGCTTT TCCGTGAGCC 
     1501 AGCGAGCAGA TGCAAGGTAC CGACACCTCA TTGCTCTTTT CACCCAAGAC GAATACTTCG 
     1561 CGATGGTGGC CGAGAACGAG TCGGAGCAGG AAAGCTGGTA CTTGCTGCTC AGCCGCCTCA 
     1621 TCCTCGAGAG CAAGCGCCGC CGCTGCGGCA CGCTCGGCGC GCAGCCGGAC GGAGAGCCGG 
     1681 CCGCGCTGGC GGCGGCAGCG GCGGCGGAGC CACCCTTCTA TAAAGATGTG TGGCAGGTAA 
     1741 TAGTCAAACC CAGGGGGCTG GGGCACAGAA AAGAGCTGAG CGGCGTGTTC CGGCTGTGTC 
     1801 TAACCGACGA GGAGGTCGTG TTTGTGAGGC TGAACACCGA AGTGGCCAGC GTGGTCGTCC 
     1861 AGCTCCTGAG CATCCGTCGC TGTGGACACT CGGAGCAGTA TTTCTTCTTG GAAGTAGGCA 
     1921 GGTCCACTGT CATCGGTCCG GGAGAGCTCT GGATGCAGGT CGATGACTGT GTGGTTGCCC 
     1981 AAAACATGCA TGAGCTGTTT TTGGAGAAGA TGAGAGCCTT GTGTGCAGAC GAATACAGAG 
     2041 CCCGCTGCCG CAGCTACAGC ATCAGCATCG GCGCCCACCT GTTAACCCTG CTGTCCGCTA 
     2101 GGAGGCACCT GGGCTTGGTG CCGCTCGAGC CGGGAGGCTG GCTCAGAAGG TCCCGCTTTG 
     2161 AGCAGTTTTG CCACCTCAGG GCCATCGGCG ACGGGGAAGA CGAGATGCTT TTCACCAGGC 
     2221 GCTTCGTAAC ACCCAGCGAG CCTGTGGCCC ACTCCAGGCG AGGAAGACTG CACCTGCCCA 
     2281 GAGGGCGCAG GTCAAGGAGA GCGGTTTCAG TGCCGGCCAG CTTTTTTCGC CGCTTAGCAC 
     2341 CCAGCCCAGC ACGTCCCCGG CACCCTGCAG AAGCCCCGAA CAATGGAGCT CGCCTGTCTT 
     2401 CTGAAGTGTC TGGTTCTGGC TCTGGCAACT TTGGGGAGGA AGGCAATCCC CAGGGCAAAG 
     2461 AAGATCAGGA AGGAAGCGGA GGTGACTACA TGCCTATGAA CAATTGGGGC TCAGGAAATG 
     2521 GCCGGGGCTC AGGAGGTGGC CAGGGCTCAA ATGGCCAAGG CTCCAGTAGC CATAGCTCGG 
     2581 GAGGAAACCA GTGTTCAGGC GAGGGACAGG GATCCCGAGG TGGTCAGGGC TCAAATGGCC 
     2641 AGGGCTCAGG AGGAAACCAG TGCTCTAGAG ATGGCCAGGG CACCGCAGGT GGGCACGGTT 
     2701 CAGGTGGTGG CCAGAGACCT GGAGGTGGGC ATGGCTCAGG TGGTGGCCAG GGACCTGGAG 
     2761 ATGGCCATGG CTCAGGTGGT GGCAAGAACT CTGGGGGGGG CAAAGGCTCA GGAAGTGGGA 
     2821 AAGGATCCGA TGGTGATGGT GAACGTGGAA AATCTCTGAA GAAAAGATCC TATTTTGGCA 
     2881 AATTAACTCA AAGCAAGCAA CAGCAAATGC CACCACCTCC ACCACCTCCT CCTCCACCCC 
     2941 CACCAGCTGG AGGAACTGGT GGAAAAGGGA AGTCTGGGGG AAGATTCAGA CTTTATTTTT 
     3001 GTGTTGACAG AGGAGCCACG AAAGAATGCA AAGAAGCCAA AGAAGTGAAA GATGCAGAGA 
     3061 TCCCAGAAGG TGCAGCTCGA GGTCCCCACA GAGCCAGAGC TTTTGATGAA GATGAGGATG 
     3121 ACCCATACGT GCCAATGAGG CCAGGGGTGG CCACCCCTCT TGTAAGCTCC AGTGATTATA 
     3181 TGCCAATGGC TCCTCAAAAT GTCTCTGCTT CAAAAAAGCG CCACTCTCGA TCCCCTTTTG 
     3241 AAGATTCAAG AGGGTACATG ATGATGTTTC CCAGAGTGAG CCCACCACCT GCTCCGAGTC 
     3301 CTCCAAAAGC ACCTGATACT AATAAAGAGG ATGACTCAAA GGACAATGAC AGTGAGAGTG 
     3361 ACTACATGTT TATGGCTCCT GGAGCCGGTG CAATTCCAAA AAACCCCAGA AATCCTCAGG 
     3421 GTGGCTCTTC CTCCAAAAGT TGGAGCTCCT ACTTCTCTCT ACCAAACCCT TTTCGGAGCT 
     3481 CACCTTTGGG ACAGAATGAC AACAGTGAGT ATGTGCCAAT GTTACCTGGA AAGTTCCTGG 
     3541 GGAGGGGCCT AGACAAAGAA GTCTCCTATA ACTGGGACCC CAAAGATGCA GCTTCAAAGC 
     3601 CTTCAGGTGA GGGATCATTC TCAAAGCCTG GAGATGGGGG ATCACCTTCA AAGCCTTCAG 
     3661 ATCATGAGCC CCCAAAGAAT AAAGCTAAGA GACCTAACCG ACTTTCTTTT ATTACAAAAG 
     3721 GATATAAAAT CAAGCCAAAA CCACAAAAGC CCACACATGA GCAGAGAGAA GCTGACAGCT 
     3781 CTAGTGACTA CGTCAACATG GACTTCACTA AAAGAGAGAG CAATACACCA GCTCCCTCTA 
     3841 CTCAAGGACT ACCAGATTCG TGGGGCATAA TTGCTGAACC CAGACAGTCA GCCTTTTCTA 
     3901 ATTATGTGAA TGTTGAGTTT GGAGTGCCAT TTCCAAATCC AGCAAACGAC CTCTCAGATC 
     3961 TTTTAAGAGC TATACCACGT GCCAACCCCT TATCTCTGGA CAGTGCTAGG TGGCCACTTC 
     4021 CTCCCCTTCC CCTCAGTGCT ACAGGTAGCA ATGCTATTGA GGAAGAGGGT GACTACATTG 
     4081 AAGTAATTTT CAACTCAGCA ATGACACCAG CCATGGCTCT TGCTGACAGT GCCATTCGCT 
     4141 ATGATGCTGA AACAGGTCGA ATCTATGTGG TCGACCCATT TTCTGAGTGC TGTATGGATA 
     4201 TTTCTCTCTC CCCCAGCCGA TGTTCTGAAC CACCACCTGT AGCTAGGCTG CTGCAGGAAG 
     4261 AAGAGCAGGA GAGAAGACGC CCACAAAGCC GTTCTCAAAG TTTCTTTGCA GCAGCCAGAG 
     4321 CCGCTGTCTC TGCTTTTCCA ACAGACAGCC TCGAGAGAGA CCTTTCCCCA TCCTCAGCCC 
     4381 CGGCTGTCGC TTCGGCTGCA GAGCCGACTT TAGCCCTCAG CCAAGTTGTA GCTGCGGCCT 
     4441 CCGCGCTCGC CGCAGCCCCG GGCATCGGCG CAGCAGCCGC AGCTGCTGGA TTTGACTCCG 
     4501 CCTCTGCCCG CTGGTTTCAA CCTGTTGCTA ATGCTGCTGA TGCCGAAGCA GTAAGGGGAG 
     4561 CCCAAGACGT TGCCGGTGGC TCGAACCCTG GAGCCCACAA CCCATCTGCA AACCTTGCCA 
     4621 GAGGTGATAA CCAGGCTGGC GGGGCTGCCG CTGCAGCTGC CGCTCCGGAA CCACCACCTC 
     4681 GCAGTCGCCG GGTGCCAAGA CCCCCGGAGA GAGAAGATTC TGACAACGAC GACGACACTC 
     4741 ACGTGAGAAT GGATTTTGCC AGACGTGATA ATCAGTTCGA CTCTCCCAAA AGAGGTCGGA 
     4801 CGCGTACGCG GCCGCTCGAG CAGAAACTCA TCTCAGAAGA GGATCTGGCA GCAAATGATA 
     4861 TCCTGGATTA CAAGGATGAC GACGATAAGG TTTAAACGGC CGGCCGCGGT CATAGCTGTT 
     4921 TCCTGAACAG ATCCCGGGTG GCATCCCTGT GACCCCTCCC CAGTGCCTCT CCTGGCCCTG 
     4981 GAAGTTGCCA CTCCAGTGCC CACCAGCCTT GTCCTAATAA AATTAAGTTG CATCATTTTG 
     5041 TCTGACTAGG TGTCCTTCTA TAATATTATG GGGTGGAGGG GGGTGGTATG GAGCAAGGGG 
     5101 CAAGTTGGGA AGACAACCTG TAGGGCCTGC GGGGTCTATT GGGAACCAAG CTGGAGTGCA 
     5161 GTGGCACAAT CTTGGCTCAC TGCAATCTCC GCCTCCTGGG TTCAAGCGAT TCTCCTGCCT 
     5221 CAGCCTCCCG AGTTGTTGGG ATTCCAGGCA TGCATGACCA GGCTCAGCTA ATTTTTGTTT 
     5281 TTTTGGTAGA GACGGGGTTT CACCATATTG GCCAGGCTGG TCTCCAACTC CTAATCTCAG 
     5341 GTGATCTACC CACCTTGGCC TCCCAAATTG CTGGGATTAC AGGCGTGAAC CACTGCTCCC 
     5401 TTCCCTGTCC TTCTGATTTT AAAATAACTA TACCAGCAGG AGGACGTCCA GACACAGCAT 
     5461 AGGCTACCTG GCCATGCCCA ACCGGTGGGA CATTTGAGTT GCTTGCTTGG CACTGTCCTC 
     5521 TCATGCGTTG GGTCCACTCA GTAGATGCCT GTTGAATTGG GTACGCGGCC AGCGGCGAGC 
     5581 GGTATCAGCT CACTCAAAGG CGGTAATACG GTTATCCACA GAATCAGGGG ATAACGCAGG 
     5641 AAAGAACATG TGAGCAAAAG GCCAGCAAAA GGCCAGGAAC CGTAAAAAGG CCGCGTTGCT 
     5701 GGCGTTTTTC CATAGGCTCC GCCCCCCTGA CGAGCATCAC AAAAATCGAC GCTCAAGTCA 
     5761 GAGGTGGCGA AACCCGACAG GACTATAAAG ATACCAGGCG TTTCCCCCTG GAAGCTCCCT 
     5821 CGTGCGCTCT CCTGTTCCGA CCCTGCCGCT TACCGGATAC CTGTCCGCCT TTCTCCCTTC 
     5881 GGGAAGCGTG GCGCTTTCTC ATAGCTCACG CTGTAGGTAT CTCAGTTCGG TGTAGGTCGT 
     5941 TCGCTCCAAG CTGGGCTGTG TGCACGAACC CCCCGTTCAG CCCGACCGCT GCGCCTTATC 
     6001 CGGTAACTAT CGTCTTGAGT CCAACCCGGT AAGACACGAC TTATCGCCAC TGGCAGCAGC 
     6061 CACTGGTAAC AGGATTAGCA GAGCGAGGTA TGTAGGCGGT GCTACAGAGT TCTTGAAGTG 
     6121 GTGGCCTAAC TACGGCTACA CTAGAAGAAC AGTATTTGGT ATCTGCGCTC TGCTGAAGCC 
     6181 AGTTACCTTC GGAAAAAGAG TTGGTAGCTC TTGATCCGGC AAACAAACCA CCGCTGGTAG 
     6241 CGGTGGTTTT TTTGTTTGCA AGCAGCAGAT TACGCGCAGA AAAAAAGGAT CTCAAGAAGA 
     6301 TCCTTTGATC TTTTCTACGG GGTCTGACGC TCAGTGGAAC GAAAACTCAC GTTAAGGGAT 
     6361 TTTGGTCATG AGATTATCAA AAAGGATCTT CACCTAGATC CTTTTAAATT AAAAATGAAG 
     6421 TTTTAAATCA ATCTAAAGTA TATATGAGTA ACCTGAGGCT ATGGCAGGGC CTGCCGCCCC 
     6481 GACGTTGGCT GCGAGCCCTG GGCCTTCACC CGAACTTGGG GGGTGGGGTG GGGAAAAGGA 
     6541 AGAAACGCGG GCGTATTGGC CCCAATGGGG TCTCGGTGGG GTATCGACAG AGTGCCAGCC 
     6601 CTGGGACCGA ACCCCGCGTT TATGAACAAA CGACCCAACA CCGTGCGTTT TATTCTGTCT 
     6661 TTTTATTGCC GTCATAGCGC GGGTTCCTTC CGGTATTGTC TCCTTCCGTG TTTCAGTTAG 
     6721 CCTCCCCCTA GGGTGGGCGA AGAACTCCAG CATGAGATCC CCGCGCTGGA GGATCATCCA 
     6781 GCCGGCGTCC CGGAAAACGA TTCCGAAGCC CAACCTTTCA TAGAAGGCGG CGGTGGAATC 
     6841 GAAATCTCGT GATGGCAGGT TGGGCGTCGC TTGGTCGGTC ATTTCGAACC CCAGAGTCCC 
     6901 GCTCAGAAGA ACTCGTCAAG AAGGCGATAG AAGGCGATGC GCTGCGAATC GGGAGCGGCG 
     6961 ATACCGTAAA GCACGAGGAA GCGGTCAGCC CATTCGCCGC CAAGCTCTTC AGCAATATCA 
     7021 CGGGTAGCCA ACGCTATGTC CTGATAGCGA TCCGCCACAC CCAGCCGGCC ACAGTCGATG 
     7081 AATCCAGAAA AGCGGCCATT TTCCACCATG ATATTCGGCA AGCAGGCATC GCCATGGGTC 
     7141 ACGACGAGAT CCTCGCCGTC GGGCATGCTC GCCTTGAGCC TGGCGAACAG TTCGGCTGGC 
     7201 GCGAGCCCCT GATGCTCTTC GTCCAGATCA TCCTGATCGA CAAGACCGGC TTCCATCCGA 
     7261 GTACGTGCTC GCTCGATGCG ATGTTTCGCT TGGTGGTCGA ATGGGCAGGT AGCCGGATCA 
     7321 AGCGTATGCA GCCGCCGCAT TGCATCAGCC ATGATGGATA CTTTCTCGGC AGGAGCAAGG 
     7381 TGAGATGACA GGAGATCCTG CCCCGGCACT TCGCCCAATA GCAGCCAGTC CCTTCCCGCT 
     7441 TCAGTGACAA CGTCGAGCAC AGCTGCGCAA GGAACGCCCG TCGTGGCCAG CCACGATAGC 
     7501 CGCGCTGCCT CGTCTTGCAG TTCATTCAGG GCACCGGACA GGTCGGTCTT GACAAAAAGA 
     7561 ACCGGGCGCC CCTGCGCTGA CAGCCGGAAC ACGGCGGCAT CAGAGCAGCC GATTGTCTGT 
     7621 TGTGCCCAGT CATAGCCGAA TAGCCTCTCC ACCCAAGCGG CCGGAGAACC TGCGTGCAAT 
     7681 CCATCTTGTT CAATCATGCG AAACGATCCT CATCCTGTCT CTTGATCGAT CTTTGCAAAA 
     7741 GCCTAGGCCT CCAAAAAAGC CTCCTCACTA CTTCTGGAAT AGCTCAGAGG CCGAGGCGGC 
     7801 CTCGGCCTCT GCATAAATAA AAAAAATTAG TCAGCCATGG GGCGGAGAAT GGGCGGAACT 
     7861 GGGCGGAGTT AGGGGCGGGA TGGGCGGAGT TAGGGGCGGG ACTATGGTTG CTGACTAATT 
     7921 GAGATGCATG CTTTGCATAC TTCTGCCTGC TGGGGAGCCT GGGGACTTTC CACACCTGGT 
     7981 TGCTGACTAA TTGAGATGCA TGCTTTGCAT ACTTCTGCCT GCTGGGGAGC CTGGGGACTT 
     8041 TCCACACCCT AACTGACACA CATTCCACAG CTGGTTCTTT CCGCCTCAGG ACTCTTCCTT 
     8101 TTTCAATATT ATTGAAGCAT TTATCAGGGT TATTGTCTCA TGAGCGGATA CATATTTGAA 
     8161 TGTATTTAGA AAAATAAACA AATAGGGGTT CCGCGCACAT TTCCCCGAAA AGTGCCACCT 
     8221 GACGCGCCCT GTAGCGGCGC ATTAAGCGCG GCGGGTGTGG TGGTTACGCG CAGCGTGACC 
     8281 GCTACACTTG CCAGCGCCCT AGCGCCCGCT CCTTTCGCTT TCTTCCCTTC CTTTCTCGCC 
     8341 ACGTTCGCCG GCTTTCCCCG TCAAGCTCTA AATCGGGGGC TCCCTTTAGG GTTCCGATTT 
     8401 AGTGCTTTAC GGCACCTCGA CCCCAAAAAA CTTGATTAGG GTGATGGTTC ACGTAGTGGG 
     8461 CCATCGCCCT GATAGACGGT TTTTCGCCCT TTGACGTTGG AGTCCACGTT CTTTAATAGT 
     8521 GGACTCTTGT TCCAAACTGG AACAACACTC AACCCTATCT CGGTCTATTC TTTTGATTTA 
     8581 TAAGGGATTT TGCCGATTTC GGCCTATTGG TTAAAAAATG AGCTGATTTA ACAAAAATTT 
     8641 AACGCGAATT TT
//