LOCUS       MR227158                7959 bp    DNA     circular  SYN 
DEFINITION  Cntn1 (Myc-DDK-tagged) - Mouse contactin 1 (Cntn1), transcript variant 2
ACCESSION   NM_007727
VERSION     NM_007727.2
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    4114..4205 
                     /label="Myc-DDK"  
     misc_feature    4235..4820 
                     /label="PolyA signal"  
     misc_feature    4292..4311 
                     /label="Restriction Enzyme"  
     misc_feature    5096..5768 
                     /label="ColE1"  
     misc_feature    6198..6992 
                     /label="Kan/Neo"  
     misc_feature    7027..7329 
                     /label="SV40 ori"  
     misc_feature    7415..7892 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    4089..4096 
                     /label="Restriction Enzyme"  
     misc_feature    1020..4089 
                     /label="Cntn1"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GAAAATGCCA TTGCTGGTCA GCCATCTCCT GCTAATCTCC TTAACATCTT 
     1081 GTCTAGGAGA CTTTACCTGG CACAGAAGAT ATGGTCATGG AGTGTCTGAG GAGGACAAAG 
     1141 GATTTGGGCC CATTTTTGAA GAGCAGCCAA TCAATACCAT TTACCCAGAA GAGTCACTGG 
     1201 AAGGAAAAGT GTCACTCAAC TGCCGGGCAC GCGCCAGCCC ATTTCCAGTT TACAAATGGA 
     1261 GAATGAATAA CGGGGACGTG GACCTGACAA ACGATCGGTA CAGTATGGTC GGAGGAAACC 
     1321 TTGTTATCAA CAACCCGGAC AAACAGAAAG ACGCTGGGGT GTACTACTGC CTGGCATCCA 
     1381 ACAACTACGG AATGGTCAGG AGCACGGAGG CAACTCTGAG CTTCGGGTAT CTCGACCCCT 
     1441 TTCCTCCCGA GGAGCGTCCG GAAGTGAAGG TGAAGGAAGG GAAGGGCATG GTGCTTCTCT 
     1501 GTGATCCTCC CTACCACTTC CCAGATGATC TTAGCTACCG CTGGCTTCTG AATGAATTTC 
     1561 CTGTATTTAT CACCATGGAT AAGCGAAGAT TTGTGTCTCA GACCAACGGC AATCTCTATA 
     1621 TCGCAAATGT CGAGTCTTCT GACAGAGGCA ACTACTCCTG CTTTGTGTCC AGTCCTTCTA 
     1681 TTACAAAGAG TGTGTTCAGC AAGTTTATTC CCCTCATTCC GATTCCTGAA CGAACAACAA 
     1741 AGCCATATCC TGCTGATATT GTGGTGCAGT TCAAGGACAT ATACACCATG ATGGGCCAAA 
     1801 ATGTGACTTT AGAGTGTTTC GCTCTAGGGA ACCCTGTTCC TGATATCCGG TGGCGGAAGG 
     1861 TGCTAGAACC AATGCCCAGC ACTGCCGAGA TAAGCACGTC AGGGGCTGTC CTCAAGATCT 
     1921 TCAACATCCA GCTAGAAGAT GAAGGCCTCT ACGAGTGTGA GGCTGAGAAC ATCCGGGGAA 
     1981 AGGACAAGCA CCAGGCAAGA ATCTATGTTC AAGCGTTTCC TGAGTGGGTA GAGCATATCA 
     2041 ATGACACTGA GGTGGACATA GGCAGTGACC TCTACTGGCC TTGCATAGCC ACAGGGAAGC 
     2101 CCATTCCTAC CATCAGGTGG CTGAAAAATG GATACTCGTA TCACAAAGGG GAACTGAGAC 
     2161 TGTATGATGT GACTTTTGAA AATGCCGGGA TGTACCAGTG CATAGCAGAA AACGCGTATG 
     2221 GGTCCATTTA TGCCAACGCT GAGCTCAAGA TCCTGGCTTT AGCTCCAACT TTTGAAATGA 
     2281 ATCCTATGAA GAAAAAGATC TTGGCTGCGA AAGGTGGAAG GGTTGTCATT GAATGTAAAC 
     2341 CCAAGGCCGC ACCAAAACCC AAGTTTTCAT GGAGCAAAGG GACAGAGTGG CTCGTCAACA 
     2401 GCAGCAGAAT ACTCATATGG GAAGATGGTA GCTTGGAAAT CAATAATATT ACAAGAAATG 
     2461 ATGGTGGAAT CTATACATGC TTTGCAGAAA ATAACAGAGG AAAAGCTAAT AGCACTGGGA 
     2521 CTCTGGTGAT CACAAATCCC ACACGAATTA TTTTGGCCCC AATCAATGCT GACATCACTG 
     2581 TTGGGGAAAA TGCCACCATG CAGTGTGCTG CGTCTTTCGA TCCTGCCTTG GACCTCACGT 
     2641 TTGTGTGGTC CTTCAATGGC TACGTGATTG ACTTTAACAA GGAAATTACG CATATCCATT 
     2701 ACCAGAGGAA TTTTATGCTC GACGCCAATG GGGAACTGCT CATCCGAAAT GCGCAGCTGA 
     2761 AACACGCAGG CCGGTACACG TGCACGGCGC AGACAATCGT GGACAATTCC TCGGCTTCCG 
     2821 CTGACCTTGT CGTTCGAGGC CCCCCAGGTC CCCCAGGAGG CCTGAGAATA GAAGACATCA 
     2881 GAGCTACCTC TGTGGCACTG ACTTGGAGCC GTGGTTCCGA CAATCACAGC CCTATCTCTA 
     2941 AATACACGAT CCAGACCAAG ACGATTCTCT CAGATGACTG GAAAGATGCG AAGACAGATC 
     3001 CCCCAATCAT TGAAGGGAAC ATGGAGTCAG CTAAAGCAGT GGATTTGATC CCTTGGATGG 
     3061 AGTATGAATT TCGAGTGGTG GCGACCAATA CTTTAGGGAC GGGAGAGCCC AGCATACCCT 
     3121 CAAACAGGAT CAAAACAGAC GGTGCTGCAC CAAATGTGGC TCCCTCAGAT GTAGGCGGTG 
     3181 GGGGCGGAAC CAACAGAGAG CTGACAATAA CGTGGGCGCC TTTGTCGAGA GAGTACCATT 
     3241 ACGGTAACAA TTTTGGTTAC ATAGTGGCGT TTAAGCCATT TGATGGAGAA GAGTGGAAGA 
     3301 AAGTGACTGT GACTAACCCA GACACTGGCC GCTACGTCCA CAAAGATGAG ACCATGACCC 
     3361 CCTCCACCGC GTTTCAAGTC AAAGTGAAAG CCTTCAACAA TAAAGGAGAT GGGCCCTACA 
     3421 GCCTCGTAGC TGTCATCAAC TCTGCACAAG ATGCTCCCAG TGAAGCCCCC ACAGAGGTAG 
     3481 GGGTCAAAGT GTTATCATCC TCTGAGATAT CTGTCCATTG GAAGCATGTG TTAGAGAAAA 
     3541 TTGTGGAAAG CTATCAGATT CGGTACTGGG CTGGGCATGA CAAAGAAGCA GCCGCACACA 
     3601 GAGTTCAGGT CACCAGCCAG GAGTACTCAG CCAGACTGGA GAACCTTCTT CCCGACACCC 
     3661 AGTACTTCAT TGAAGTGGGA GCCTGCAACA GTGCTGGATG CGGACCTTCC AGTGATGTGA 
     3721 TCGAGACCTT CACCAGAAAA GCCCCTCCTA GCCAACCACC AAGGATCATC AGTTCGGTGA 
     3781 GATCTGGCTC ACGATATATA ATCACATGGG ATCACGTTGT GGCACTATCC AATGAATCTA 
     3841 CTGTGACAGG CTACAAGATA CTCTATAGAC CGGATGGCCA ACATGACGGC AAGCTGTTCT 
     3901 CAACCCATAA ACACTCCATA GAAGTCCCCA TCCCCAGAGA CGGAGAGTAT GTTGTCGAGG 
     3961 TTCGAGCACA CAGCGACGGG GGAGACGGAG TTGTGTCTCA AGTCAAAATT TCAGGCGTGT 
     4021 CCACGCTGTC TTCGAGCCTC CTCAGCTTGC TCCTGCCCTC CCTTGGCTTT CTTGTCTACT 
     4081 CGGAATTCAG CGGACCGAGC GGACCGACGC GTACGCGGCC GCTCGAGCAG AAACTCATCT 
     4141 CAGAAGAGGA TCTGGCAGCA AATGATATCC TGGATTACAA GGATGACGAC GATAAGGTTT 
     4201 AAACGGCCGG CCGCGGTCAT AGCTGTTTCC TGAACAGATC CCGGGTGGCA TCCCTGTGAC 
     4261 CCCTCCCCAG TGCCTCTCCT GGCCCTGGAA GTTGCCACTC CAGTGCCCAC CAGCCTTGTC 
     4321 CTAATAAAAT TAAGTTGCAT CATTTTGTCT GACTAGGTGT CCTTCTATAA TATTATGGGG 
     4381 TGGAGGGGGG TGGTATGGAG CAAGGGGCAA GTTGGGAAGA CAACCTGTAG GGCCTGCGGG 
     4441 GTCTATTGGG AACCAAGCTG GAGTGCAGTG GCACAATCTT GGCTCACTGC AATCTCCGCC 
     4501 TCCTGGGTTC AAGCGATTCT CCTGCCTCAG CCTCCCGAGT TGTTGGGATT CCAGGCATGC 
     4561 ATGACCAGGC TCAGCTAATT TTTGTTTTTT TGGTAGAGAC GGGGTTTCAC CATATTGGCC 
     4621 AGGCTGGTCT CCAACTCCTA ATCTCAGGTG ATCTACCCAC CTTGGCCTCC CAAATTGCTG 
     4681 GGATTACAGG CGTGAACCAC TGCTCCCTTC CCTGTCCTTC TGATTTTAAA ATAACTATAC 
     4741 CAGCAGGAGG ACGTCCAGAC ACAGCATAGG CTACCTGGCC ATGCCCAACC GGTGGGACAT 
     4801 TTGAGTTGCT TGCTTGGCAC TGTCCTCTCA TGCGTTGGGT CCACTCAGTA GATGCCTGTT 
     4861 GAATTGGGTA CGCGGCCAGC GGCGAGCGGT ATCAGCTCAC TCAAAGGCGG TAATACGGTT 
     4921 ATCCACAGAA TCAGGGGATA ACGCAGGAAA GAACATGTGA GCAAAAGGCC AGCAAAAGGC 
     4981 CAGGAACCGT AAAAAGGCCG CGTTGCTGGC GTTTTTCCAT AGGCTCCGCC CCCCTGACGA 
     5041 GCATCACAAA AATCGACGCT CAAGTCAGAG GTGGCGAAAC CCGACAGGAC TATAAAGATA 
     5101 CCAGGCGTTT CCCCCTGGAA GCTCCCTCGT GCGCTCTCCT GTTCCGACCC TGCCGCTTAC 
     5161 CGGATACCTG TCCGCCTTTC TCCCTTCGGG AAGCGTGGCG CTTTCTCATA GCTCACGCTG 
     5221 TAGGTATCTC AGTTCGGTGT AGGTCGTTCG CTCCAAGCTG GGCTGTGTGC ACGAACCCCC 
     5281 CGTTCAGCCC GACCGCTGCG CCTTATCCGG TAACTATCGT CTTGAGTCCA ACCCGGTAAG 
     5341 ACACGACTTA TCGCCACTGG CAGCAGCCAC TGGTAACAGG ATTAGCAGAG CGAGGTATGT 
     5401 AGGCGGTGCT ACAGAGTTCT TGAAGTGGTG GCCTAACTAC GGCTACACTA GAAGAACAGT 
     5461 ATTTGGTATC TGCGCTCTGC TGAAGCCAGT TACCTTCGGA AAAAGAGTTG GTAGCTCTTG 
     5521 ATCCGGCAAA CAAACCACCG CTGGTAGCGG TGGTTTTTTT GTTTGCAAGC AGCAGATTAC 
     5581 GCGCAGAAAA AAAGGATCTC AAGAAGATCC TTTGATCTTT TCTACGGGGT CTGACGCTCA 
     5641 GTGGAACGAA AACTCACGTT AAGGGATTTT GGTCATGAGA TTATCAAAAA GGATCTTCAC 
     5701 CTAGATCCTT TTAAATTAAA AATGAAGTTT TAAATCAATC TAAAGTATAT ATGAGTAACC 
     5761 TGAGGCTATG GCAGGGCCTG CCGCCCCGAC GTTGGCTGCG AGCCCTGGGC CTTCACCCGA 
     5821 ACTTGGGGGG TGGGGTGGGG AAAAGGAAGA AACGCGGGCG TATTGGCCCC AATGGGGTCT 
     5881 CGGTGGGGTA TCGACAGAGT GCCAGCCCTG GGACCGAACC CCGCGTTTAT GAACAAACGA 
     5941 CCCAACACCG TGCGTTTTAT TCTGTCTTTT TATTGCCGTC ATAGCGCGGG TTCCTTCCGG 
     6001 TATTGTCTCC TTCCGTGTTT CAGTTAGCCT CCCCCTAGGG TGGGCGAAGA ACTCCAGCAT 
     6061 GAGATCCCCG CGCTGGAGGA TCATCCAGCC GGCGTCCCGG AAAACGATTC CGAAGCCCAA 
     6121 CCTTTCATAG AAGGCGGCGG TGGAATCGAA ATCTCGTGAT GGCAGGTTGG GCGTCGCTTG 
     6181 GTCGGTCATT TCGAACCCCA GAGTCCCGCT CAGAAGAACT CGTCAAGAAG GCGATAGAAG 
     6241 GCGATGCGCT GCGAATCGGG AGCGGCGATA CCGTAAAGCA CGAGGAAGCG GTCAGCCCAT 
     6301 TCGCCGCCAA GCTCTTCAGC AATATCACGG GTAGCCAACG CTATGTCCTG ATAGCGATCC 
     6361 GCCACACCCA GCCGGCCACA GTCGATGAAT CCAGAAAAGC GGCCATTTTC CACCATGATA 
     6421 TTCGGCAAGC AGGCATCGCC ATGGGTCACG ACGAGATCCT CGCCGTCGGG CATGCTCGCC 
     6481 TTGAGCCTGG CGAACAGTTC GGCTGGCGCG AGCCCCTGAT GCTCTTCGTC CAGATCATCC 
     6541 TGATCGACAA GACCGGCTTC CATCCGAGTA CGTGCTCGCT CGATGCGATG TTTCGCTTGG 
     6601 TGGTCGAATG GGCAGGTAGC CGGATCAAGC GTATGCAGCC GCCGCATTGC ATCAGCCATG 
     6661 ATGGATACTT TCTCGGCAGG AGCAAGGTGA GATGACAGGA GATCCTGCCC CGGCACTTCG 
     6721 CCCAATAGCA GCCAGTCCCT TCCCGCTTCA GTGACAACGT CGAGCACAGC TGCGCAAGGA 
     6781 ACGCCCGTCG TGGCCAGCCA CGATAGCCGC GCTGCCTCGT CTTGCAGTTC ATTCAGGGCA 
     6841 CCGGACAGGT CGGTCTTGAC AAAAAGAACC GGGCGCCCCT GCGCTGACAG CCGGAACACG 
     6901 GCGGCATCAG AGCAGCCGAT TGTCTGTTGT GCCCAGTCAT AGCCGAATAG CCTCTCCACC 
     6961 CAAGCGGCCG GAGAACCTGC GTGCAATCCA TCTTGTTCAA TCATGCGAAA CGATCCTCAT 
     7021 CCTGTCTCTT GATCGATCTT TGCAAAAGCC TAGGCCTCCA AAAAAGCCTC CTCACTACTT 
     7081 CTGGAATAGC TCAGAGGCCG AGGCGGCCTC GGCCTCTGCA TAAATAAAAA AAATTAGTCA 
     7141 GCCATGGGGC GGAGAATGGG CGGAACTGGG CGGAGTTAGG GGCGGGATGG GCGGAGTTAG 
     7201 GGGCGGGACT ATGGTTGCTG ACTAATTGAG ATGCATGCTT TGCATACTTC TGCCTGCTGG 
     7261 GGAGCCTGGG GACTTTCCAC ACCTGGTTGC TGACTAATTG AGATGCATGC TTTGCATACT 
     7321 TCTGCCTGCT GGGGAGCCTG GGGACTTTCC ACACCCTAAC TGACACACAT TCCACAGCTG 
     7381 GTTCTTTCCG CCTCAGGACT CTTCCTTTTT CAATATTATT GAAGCATTTA TCAGGGTTAT 
     7441 TGTCTCATGA GCGGATACAT ATTTGAATGT ATTTAGAAAA ATAAACAAAT AGGGGTTCCG 
     7501 CGCACATTTC CCCGAAAAGT GCCACCTGAC GCGCCCTGTA GCGGCGCATT AAGCGCGGCG 
     7561 GGTGTGGTGG TTACGCGCAG CGTGACCGCT ACACTTGCCA GCGCCCTAGC GCCCGCTCCT 
     7621 TTCGCTTTCT TCCCTTCCTT TCTCGCCACG TTCGCCGGCT TTCCCCGTCA AGCTCTAAAT 
     7681 CGGGGGCTCC CTTTAGGGTT CCGATTTAGT GCTTTACGGC ACCTCGACCC CAAAAAACTT 
     7741 GATTAGGGTG ATGGTTCACG TAGTGGGCCA TCGCCCTGAT AGACGGTTTT TCGCCCTTTG 
     7801 ACGTTGGAGT CCACGTTCTT TAATAGTGGA CTCTTGTTCC AAACTGGAAC AACACTCAAC 
     7861 CCTATCTCGG TCTATTCTTT TGATTTATAA GGGATTTTGC CGATTTCGGC CTATTGGTTA 
     7921 AAAAATGAGC TGATTTAACA AAAATTTAAC GCGAATTTT
//